Early detection and biological control for peach crown gall prevention in the nurseries. (Abstract/Poster in convegno)

Type
Label
  • Early detection and biological control for peach crown gall prevention in the nurseries. (Abstract/Poster in convegno) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Alternative label
  • RAIO A., PUOPOLO G., PELUSO G., ZOINA A. (2005)
    Early detection and biological control for peach crown gall prevention in the nurseries.
    in SIPaV XII National Meeting, Reggio Calabria
    (literal)
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  • RAIO A., PUOPOLO G., PELUSO G., ZOINA A. (literal)
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  • Journal of Plant Pathology, 87(4, Special issue), 303. SIPaV XII National Meeting, Reggio Calabria, 29 September - 1 October 2005. (literal)
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  • Crown gall, caused by Agrobacterium spp., can be considered a main limiting factor for peach cultivation in the nurseries. Trials were carried out on the early detection of crown gall and the evaluation of biocontrol with the antagonist K84. A PCR-based protocol that allows detection of latent infections by tumorigenic agrobacteria in peach plants was developed. Total DNA was extracted by a rapid procedure and the highly conserved sequences of the virulence (vir) region of Ti plasmid of Agrobacterium spp. were amplified. Agrobacteria were detected in the roots and up to 20 cm of the stems of artificially inoculated symptomless peach plants. The procedure developed in this study may be a useful tool for the certification of plant propagation material and for epidemiological studies on crown gall disease. The efficacy of the antagonist Agrobacterium rhizogenes strain K84 in preventing crown gall of peach was monitored for several years in some nurseries of Southern Italy. In 2001, tumors were observed in K84 protected plants grown in a nursery of the Campania region (southern Italy). Tumorigenic and K84 insensitive strains, originated by plasmid exchange between K84 and autochthonous tumorigenic agrobacteria, were found in tumors. These agrobacteria also showed high rhizosphere competence and may represent a real threat for biocontrol implementation. Although strain K1026 (a K84 derivative) could prevent the selection of dangerous transconjugant agrobacteria, its use is not allowed in the UE for it is regarded as a genetically modified microorganism. (literal)
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  • - Raio A., CNR, Ist. Protezione Piante - Sez. di Firenze - Puopolo G., Zoina A., Dipartimento di Arboricoltura, Botanica e Patologia Vegetale, Università Federico II (NA) (literal)
Titolo
  • Early detection and biological control for peach crown gall prevention in the nurseries. (literal)
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