Cloning, epression, purification, crystallization and preliminary X-ray crystallographic analyis of C-12 Hydroxylase EryK from Saccharoplyspora erythraea (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Cloning, epression, purification, crystallization and preliminary X-ray crystallographic analyis of C-12 Hydroxylase EryK from Saccharoplyspora erythraea (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.2174/092986608786071201 (literal)

Alternative label

• Savino,C.; Miele,A.E.; Sciara,G.; Kendrew,S.G.; Vallone,B. (2008)
  Cloning, epression, purification, crystallization and preliminary X-ray crystallographic analyis of C-12 Hydroxylase EryK from Saccharoplyspora erythraea
  in Protein and peptide letters (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Savino,C.; Miele,A.E.; Sciara,G.; Kendrew,S.G.; Vallone,B. (literal)

Pagina inizio

• 1138 (literal)

Pagina fine

• 1141 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 15 (literal)

Rivista

• Protein and peptide letters (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 4 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 10 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Univ Roma La Sapienza, Ist Biol & Patol Mol, CNR, I-00185 Rome, Italy 2. Univ Roma La Sapienza, Dipartimento Sci Biochim, I-00185 Rome, Italy 3. Biot Technol Ltd, Saffron Walden CB10 1XL, Essex, England (literal)

Titolo

• Cloning, epression, purification, crystallization and preliminary X-ray crystallographic analyis of C-12 Hydroxylase EryK from Saccharoplyspora erythraea (literal)

Abstract

• Erythromycin A is produced by Saccharopolyspora erythraea via a secondary metabolic pathway using several steps including glycosylations and hydroxylations of the first macrolide intermediate 6-deoxyerythronolide B. Erythromycin C-12 hydroxylase (CYP113A1), the P450 cytochrome active in the final stages of erythromycin biosynthesis, was cloned and expressed in E. coli. Different crystal forms were harvested from distinct crystallization conditions: two ligand-free forms, one substrate bound and two inhibitors-bound. All crystals belong either to the monoclinc P2(1) or to the orthorhombic P2(1)2(1)2(1) space groups, and exhibit diffraction limits ranging from 2.3 to 1.6 angstrom. The structures will be determined by molecular replacement. (literal)

Prodotto di

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Biologia strutturale e bioinformatica: struttura-funzione, dinamica e riconoscimento in proteine (SV.P14.008.001) (Modulo)

Autore CNR

• CARMELINDA SAVINO (Persona)

Insieme di parole chiave

• Parole chiave di "Cloning, epression, purification, crystallization and preliminary X-ray crystallographic analyis of C-12 Hydroxylase EryK from Saccharoplyspora erythraea" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Biologia strutturale e bioinformatica: struttura-funzione, dinamica e riconoscimento in proteine (SV.P14.008.001) (Modulo)

Autore CNR di

• CARMELINDA SAVINO (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Protein and peptide letters (Rivista)

Insieme di parole chiave di

• Parole chiave di "Cloning, epression, purification, crystallization and preliminary X-ray crystallographic analyis of C-12 Hydroxylase EryK from Saccharoplyspora erythraea" (Insieme di parole chiave)