The Drosophila Mre11/Rad50 Complex is required to prevent both telomeric fusion and chromosome breakage. (Articolo in rivista)

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  • The Drosophila Mre11/Rad50 Complex is required to prevent both telomeric fusion and chromosome breakage. (Articolo in rivista) (literal)
Anno
  • 2004-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.cub.2004.07.019 (literal)
Alternative label
  • Ciapponi L; Cenci G; Ducau J; Flores C; Johnson-Schlitz D; Gorski MM; Engels WR; Gatti M. (2004)
    The Drosophila Mre11/Rad50 Complex is required to prevent both telomeric fusion and chromosome breakage.
    in Current biology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Ciapponi L; Cenci G; Ducau J; Flores C; Johnson-Schlitz D; Gorski MM; Engels WR; Gatti M. (literal)
Pagina inizio
  • 1360 (literal)
Pagina fine
  • 1366 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 14 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 7 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Ciapponi L; Gatti M: Dipartimento di Genetica e Biologia Molecolare and Istituto di Biologia e Patologia Molecolari del CNR, Università di Roma \"La Sapienza\" Cenci G.: Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università di Lecce, Lecce Ducau J; Flores C; Johnson-Schlitz D; Engels WR.: Laboratory of Genetics, University of Wisconsin, Madison, WI 53706, USA. Gorski MM.:Department of Toxicogenetics, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands (literal)
Titolo
  • The Drosophila Mre11/Rad50 Complex is required to prevent both telomeric fusion and chromosome breakage. (literal)
Abstract
  • The MRN complex consists of the two evolutionarily conserved components Mre11 and Rad50 and the third less conserved component Nbs1/Xrs2. This complex mediates telomere maintenance plus a variety of functions in response to DNA double strand breaks, including homologous recombination, non-homologous end joining (NHEJ) and activation of DNA damage checkpoints. Mutations in the Mre11 gene cause the human ataxia-telangiectasia like disorder (ATDL). Here we show that null mutations in the Drosophila mre11 and rad50 genes cause both telomeric fusion and chromosome breakage. Moreover, we demonstrate that these mutations are in the same epistasis group required for telomere capping and mitotic chromosome integrity. Using an antibody against Rad50, we show that this protein is uniformly distributed along mitotic chromosomes, and that Rad50 is unstable in the absence of its binding partner Mre11. To define the roles of rad50 and mre11 in telomere protection, mutant chromosome preparations were immunostained for both HP1 and HOAP, two proteins that protect Drosophila telomeres from fusion. Cytological analysis revealed that mutations in rad50 and mre11 drastically reduce accumulation of HOAP and HP1 at telomeres. This suggests that MRN complex protects Drosophila telomeres by facilitating recruitment of HOAP and HP1 at chromosome ends. (literal)
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