Novel activation domain derived from Che-1 cofactor coupled with the artificial protein Jazz drives utrophin upregulation. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Novel activation domain derived from Che-1 cofactor coupled with the artificial protein Jazz drives utrophin upregulation. (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Alternative label

• Desantis A; Onori A; Di Certo MG; Mattei E; Fanciulli M; Passananti C; Corbi N. (2008)
  Novel activation domain derived from Che-1 cofactor coupled with the artificial protein Jazz drives utrophin upregulation.
  in Neuromuscular disorders
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Desantis A; Onori A; Di Certo MG; Mattei E; Fanciulli M; Passananti C; Corbi N. (literal)

Rivista

• Neuromuscular disorders (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Department of Experimental Medicine, University of L'Aquila, Via Vetoio, Coppito 2, 67100 L'Aquila, Italy;Istituto di Biologia e Patologia Molecolari, CNR, c/o Regina Elena Cancer Institute, Via delle Messi d'Oro 156, 00158 Rome, Italy;Istituto di Neurobiologia e Medicina Molecolare, CNR, c/o Fondazione Santa Lucia/EBRI, Via del Fosso di Fiorano 64/65, 00143 Rome, Italy;Laboratory \"B\", Regina Elena Cancer Institute, Via delle Messi d'Oro 156, 00158 Rome, Italy-AIRC-Roman Oncogenomic Center (ROC), Via delle Messi d'Oro 156, 00158 Rome, Italy;Istituto di Biologia e Patologia Molecolari, CNR, c/o Regina Elena Cancer Institute, Via delle Messi d'Oro 156, 00158 Rome, Italy;Istituto di Biologia e Patologia Molecolari, CNR, c/o Regina Elena Cancer Institute, Via delle Messi d'Oro 156, 00158 Rome, Italy. (literal)

Titolo

• Novel activation domain derived from Che-1 cofactor coupled with the artificial protein Jazz drives utrophin upregulation. (literal)

Abstract

• Our aim is to upregulate the expression level of the dystrophin related gene utrophin in Duchenne muscular dystrophy, thus complementing the lack of dystrophin functions. To this end we have engineered synthetic zinc finger based transcription factors. We have previously shown that the artificial three zinc finger protein named Jazz fused with the Vp16 activation domain, is able to bind utrophin promoter A and to increase the endogenous level of utrophin in transgenic mice. Here, we report on an innovative artificial protein, named CJ7, that consists of Jazz DNA binding domain fused to a novel activation domain derived from the regulatory multivalent adaptor protein Che-1/AATF. This transcriptional activation domain is 100 amino acids in size and it is very powerful as compared to the Vp16 activation domain. We show that CJ7 protein efficiently promotes transcription and accumulation of the acetylated form of histone H3 on the genomic utrophin promoter locus. (literal)

Prodotto di

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Regolazione dell'espressione genica e sua integrazione con la rete di segnalazione cellulare (SV.P13.005.001) (Modulo)

Autore CNR

• ANNALISA ONORI (Persona)
• CLAUDIO PASSANANTI (Unità di personale interno)
• NICOLETTA CORBI (Persona)

Incoming links:

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Regolazione dell'espressione genica e sua integrazione con la rete di segnalazione cellulare (SV.P13.005.001) (Modulo)

Autore CNR di

• CLAUDIO PASSANANTI (Unità di personale interno)
• ANNALISA ONORI (Persona)
• NICOLETTA CORBI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Neuromuscular disorders (Rivista)