Functional characterization of XendoU, the endoribonuclease involved in small nucleolar RNA biosynthesis. (Articolo in rivista)

Type
Label
  • Functional characterization of XendoU, the endoribonuclease involved in small nucleolar RNA biosynthesis. (Articolo in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Alternative label
  • Gioia U; Laneve P; Dlakic M; Arceci M; Bozzoni I; Caffarelli E (2005)
    Functional characterization of XendoU, the endoribonuclease involved in small nucleolar RNA biosynthesis.
    in The Journal of biological chemistry (CD-ROM)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Gioia U; Laneve P; Dlakic M; Arceci M; Bozzoni I; Caffarelli E (literal)
Pagina inizio
  • 18996 (literal)
Pagina fine
  • 19002 (literal)
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  • 280 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Gioia U, Institute Pasteur-Fondazione Cenci Bolognetti, Department of Genetics and Molecular Biology, University \"La Sapienza\",Rome Laneve P, Institute of Molecular Biology and Pathology, National Research Council, Rome Dlakic M, Department of Microbiology, Montana State University, Bozeman, Montana Arceci M, Institute of Molecular Biology and Pathology, National Research Council, Rome Bozzoni I, Institute Pasteur-Fondazione Cenci Bolognetti, Department of Genetics and Molecular Biology, University \"La Sapienza\",Rome Caffarelli E, Institute of Molecular Biology and Pathology, National Research Council, Rome (literal)
Titolo
  • Functional characterization of XendoU, the endoribonuclease involved in small nucleolar RNA biosynthesis. (literal)
Abstract
  • XendoU is the endoribonuclease involved in the biosynthesis of a specific subclass of Xenopus laevis intron-encoded small nucleolar RNAs. XendoU has no homology to any known cellular RNase, although it has sequence similarity with proteins tentatively annotated as serine proteases. It has been recently shown that XendoU represents the cellular counterpart of a nidovirus replicative endoribonuclease (NendoU), which plays a critical role in viral replication and transcription. In this paper, we combined prediction and experimental data to define the amino acid residues directly involved in XendoU catalysis. Specifically, we find that XendoU residues Glu-161, Glu-167, His-162, His-178, and Lys-224 are essential for RNA cleavage, which occurs in the presence of manganese ions. Furthermore, we identified the RNA sequence required for XendoU binding and showed that the formation of XendoU-RNA complex is Mn2+-independent. (literal)
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