The contribution of peroxynitrite generation in HIV replication in human primary macrophages (Articolo in rivista)

Type
Label
  • The contribution of peroxynitrite generation in HIV replication in human primary macrophages (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1186/1742-4690-4-76 (literal)
Alternative label
  • Stefano Aquaro 1,2; Carolina Muscoli 3,4; Alessandro Ranazzi 1; Michela Pollicita 1; Teresa Granato 5; Laura Masuelli 1; Andrea Modesti 1; Carlo Federico Perno 1; Vincenzo Mollace 3,4,6 (2007)
    The contribution of peroxynitrite generation in HIV replication in human primary macrophages
    in Retrovirology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Stefano Aquaro 1,2; Carolina Muscoli 3,4; Alessandro Ranazzi 1; Michela Pollicita 1; Teresa Granato 5; Laura Masuelli 1; Andrea Modesti 1; Carlo Federico Perno 1; Vincenzo Mollace 3,4,6 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Article number 76. Official impact factor di Retrovirology 5.24 (literal)
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  • 4 (literal)
Rivista
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  • 10 (literal)
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  • ISI Web of Science (WOS) (literal)
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  • 1 Department of Experimental Medicine and Biochemical Sciences, University of Tor Vergata, Rome, Italy, 2 Department of Pharmaco-Biology, University of Calabria, Rende(CS), Italy, 3 Faculty of Pharmacy, University of Catanzaro \"Magna Graecia\", Roccelletta di Borgia, Catanzaro, Italy, 4 San Raffaele Pisana IRCCS, Rome, Italy, 5 CNR - IBPM, Rome, Italy 6 Istitute Mondino-Tor Vergata, Rome, Italy (literal)
Titolo
  • The contribution of peroxynitrite generation in HIV replication in human primary macrophages (literal)
Abstract
  • BACKGROUND: Monocytes/Macrophages (M/M) play a pivotal role as a source of virus during the whole course of HIV-1 infection. Enhanced oxidative stress is involved in the pathogenesis of HIV-1 infection. HIV-1 regulatory proteins induce a reduction of the expression and the activity of MnSOD, the mitochondrial isoform leading to a sustained generation of superoxide anions and peroxynitrite that represent important mediators of HIV-1 replication in M/M. MnTBAP (Mn(III)tetrakis(4-benzoic acid)porphrin chloride), a synthetic peroxynitrite decomposition catalyst, reduced oxidative stress subsequent to peroxynitrite generation. RESULTS: Virus production was assessed by p24 ELISA, western blot, and electron microscopy during treatment with MnTBAP. MnTBAP treatment showed a reduction of HIV-1 replication in both acutely and chronically infected M/M: 99% and 90% inhibition of p24 released in supernatants compared to controls, respectively. Maturation of p55 and p24 was strongly inhibited by MnTBAP in both acutely and chronically infected M/M. EC50 and EC90 are 3.7 (+/- 0.05) microM and 19.5 (+/- 0.5) microM, in acutely infected M/M; 6.3 (+/- 0.003) microM and 30 (+/- 0.6) microM, in chronically infected M/M. In acutely infected peripheral blood limphocytes (PBL), EC50 and EC90 are 7.4 (+/- 0.06) microM and of 21.3 (+/- 0.6) microM, respectively. Treatment of acutely-infected M/M with MnTBAP inhibited the elevated levels of malonildialdehyde (MDA) together with the nitrotyrosine staining observed during HIV-1 replication. MnTBAP strongly reduced HIV-1 particles in infected M/M, as shown by electron microscopy. Moreover, in presence of MnTBAP, HIV-1 infectivity was reduced of about 1 log compared to control. CONCLUSION: Results support the role of superoxide anions in HIV-1 replication in M/M and suggest that MnTBAP may counteract HIV-1 replication in combination with other antiretroviral treatments (literal)
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