The role of centrosomes and astral microtubules during asymmetric division of Drosophila neuroblasts (Articolo in rivista)

Type
Label
  • The role of centrosomes and astral microtubules during asymmetric division of Drosophila neuroblasts (Articolo in rivista) (literal)
Anno
  • 2001-01-01T00:00:00+01:00 (literal)
Alternative label
  • Giansanti MG 1, Gatti M 1, 2, Bonaccorsi S. 1 (2001)
    The role of centrosomes and astral microtubules during asymmetric division of Drosophila neuroblasts
    in Development (Camb.)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Giansanti MG 1, Gatti M 1, 2, Bonaccorsi S. 1 (literal)
Pagina inizio
  • 1137 (literal)
Pagina fine
  • 1145 (literal)
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  • This paper provides an original model on the mechanisms underlying unequal cytokinesis in Drosophila neuroblasts. This model also applies to the many cell types that divide asymmetrically. This paper is published in a highly valued journal. (literal)
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  • 128 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • M. Gatti a disposizione dell'IBPM (literal)
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  • 9 (literal)
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  • Our results indicate that in Drosophila neuroblasts (NBs) and ganglion mother cells (GMCs), the molecular signals for cytokinesis are specifically provided by the central spindle midzone, where they accumulate in the complete absence of asters. Our observations suggest a model for unequal cytokinesis in Drosophila NBs. We propose that the GMC pole possesses a specialized cortical site that interacts with the GMC nucleus, anchoring it to the cell pole. The GMC nucleus would in turn interact with the central spindle, shifting its position towards the GMC cell pole and leading to unequal cytokinesis. (literal)
Note
  • ISI Web of Science (WOS) (literal)
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  • 1. CNR (IBPM, ex Centro di Genetica Evoluzionistica), 2. UNI Sapienza (Dipartimento Genetica e Biologia Molecolare) (literal)
Titolo
  • The role of centrosomes and astral microtubules during asymmetric division of Drosophila neuroblasts (literal)
Abstract
  • Drosophila neuroblasts are stem cells that divide asymmetrically to produce another large neuroblast and a smaller ganglion mother cell (GMC). During neuroblast division, several cell fate determinants, such as Miranda, Prospero and Numb, are preferentially segregated into the GMC, ensuring its correct developmental fate. The accurate segregation of these determinants relies on proper orientation of the mitotic spindle within the dividing neuroblast, and on the correct positioning of the cleavage plane. In this study we have analyzed the role of centrosomes and astral microtubules in neuroblast spindle orientation and cytokinesis. We examined neuroblast division in asterless (asl) mutants, which, although devoid of functional centrosomes and astral microtubules, form well-focused anastral spindles that undergo anaphase and telophase. We show that asl neuroblasts assemble a normal cytokinetic ring around the central spindle midzone and undergo unequal cytokinesis. Thus, astral microtubules are not required for either signaling or positioning cytokinesis in Drosophila neuroblasts. Our results indicate that the cleavage plane is dictated by the positioning of the central spindle midzone within the cell, and suggest a model on how the central spindle attains an asymmetric position during neuroblast mitosis. We have also analyzed the localization of Miranda during mitotic division of asl neuroblasts. This protein accumulates in morphologically regular cortical crescents but these crescents are mislocalized with respect to the spindle orientation. This suggests that astral microtubules mediate proper spindle rotation during neuroblast division. (literal)
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