Resveratrol affects in a different way primary versus fixed DNA damage induced by H2O2 in mammalian cells in vitro. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Resveratrol affects in a different way primary versus fixed DNA damage induced by H2O2 in mammalian cells in vitro. (Articolo in rivista) (literal)

Anno

• 2002-01-01T00:00:00+01:00 (literal)

Alternative label

• De Salvia R., Festa F., Ricordy R., Perticone P., Cozzi R. (2002)
  Resveratrol affects in a different way primary versus fixed DNA damage induced by H2O2 in mammalian cells in vitro.
  
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• De Salvia R., Festa F., Ricordy R., Perticone P., Cozzi R. (literal)

Pagina inizio

• 12 (literal)

Pagina fine

• 9 (literal)

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• Resveratrol, in our experimental conditions, seems to have both an antioxidant and a pro-oxidant activity. This behaviour is substantially consequent on the type of treatment performed and is common to many natural antioxidants (for example Ascorbic acid). On the other hand the ability of Resveratrol to induce ‘per se’ oxidant complexes is well documented. We can conclude that the mechanism of Resveratrol action appears to be very complex and elusive, depending from doses applied, supply conditions, test systems employed and end-points observed. Especially this last aspect needs to be carefully considered taking into account its capacity in modulating cell cycle and triggering more damaged cells to undergoing apoptosis . This last activity could eliminate from cell population cells heavily damaged avoiding their detection. (literal)

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• 135 (literal)

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• The aim of our work was to study the antioxidant activity eventually exerted by Resveratrol against reactive oxygen species (ROS) induced by H2O2 treatment, in Chinese hamster ovary (CHO) cells. We focused our attention on two major potential mechanisms: (a) scavenging activity and interference with oxidative metabolism (b) pro-oxidant effect. The following three targets were analysed: (i) intracellular oxidation (cytofluorimetric analysis of cellular fluorescence), (ii) primary DNA damage (single cell gel electrophoresis), (iii) fixed DNA damage (chromosomal aberrations). (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1- CNR (Istituto di Biologia e Patologia Molecolari - Sezione di Genetica) 2- UNI Roma Tre (Dipartimento di Biologia) (literal)

Titolo

• Resveratrol affects in a different way primary versus fixed DNA damage induced by H2O2 in mammalian cells in vitro. (literal)

Abstract

• Resveratrol (3,5,4'-trihydroxystilbene) is a natural occurring molecule, synthesized by plants in response to different stresses. Recent literature data seem to converge in indicating Resveratrol as an agent possessing protective effects against oxidative stresses through different mechanisms. Furthermore conflicting data are present in relation to its activity of free radical scavenger. Here we studied the antioxidant activity actually exerted by the agent against reactive oxygen species induced by H(2)O(2) treatments in CHO cells. Our attention has been focused on two major potential mechanisms: scavenging activity and interference with oxidative metabolism, by the analysis of three important targets: intracellular oxidation (Dichlorofluorescein Test), primary DNA damage (Comet Assay) and fixed DNA damage (chromosomal aberrations). Cells were treated with a single H(2)O(2) dose (2x10(-4) M) in order to induce Reactive Oxygen Species and than challenged with Resveratrol to test its ability in modulating damage. Two experimental protocols have been applied: (i) simultaneous treatment and (ii) a 3 h Resveratrol pre-treatment. In our experimental conditions Resveratrol does not appear able, 'per se', to induce primary DNA damage whereas a slight increase in endogenous oxidation and chromosomal aberrations at the highest dose have to be noticed. In combined treatments the molecule appears to differently affect primary and fixed DNA damage. (literal)

Prodotto di

• Institute of molecular biology and pathology (IBPM) (Istituto)

Autore CNR

• PAOLO PERTICONE (Unità di personale interno)
• RUGGERO RICORDY (Persona)
• ROSELLA DE SALVIA (Persona)

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• Parole chiave di "Resveratrol affects in a different way primary versus fixed DNA damage induced by H2O2 in mammalian cells in vitro." (Insieme di parole chiave)

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Autore CNR di

• RUGGERO RICORDY (Persona)
• PAOLO PERTICONE (Unità di personale interno)
• ROSELLA DE SALVIA (Persona)

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)

Insieme di parole chiave di

• Parole chiave di "Resveratrol affects in a different way primary versus fixed DNA damage induced by H2O2 in mammalian cells in vitro." (Insieme di parole chiave)