Engineering a stem cell loaded injectable nucleus pulposus substitute (Comunicazione a convegno)

Type

• Comunicazione a convegno (Classe)
• Prodotto della ricerca (Classe)

Label

• Engineering a stem cell loaded injectable nucleus pulposus substitute (Comunicazione a convegno) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Alternative label

• F. Causa, A. Gloria, A. Borzacchiello, L. Di Silvio, N. Gurav, C. Longinotti, D. Bellini, F. Piccolo, C. Di Bartolo, L. Ambrosio (2006)
  Engineering a stem cell loaded injectable nucleus pulposus substitute
  in 20th European Conference on Biomaterials, Nantes
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• F. Causa, A. Gloria, A. Borzacchiello, L. Di Silvio, N. Gurav, C. Longinotti, D. Bellini, F. Piccolo, C. Di Bartolo, L. Ambrosio (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• The aim of this research was to engineer an injectable hyaluronan-based NP substitute, with similar viscoelastic properties as the NP and the potential to incorporate bone marrow stem cells. Thus the HA providing a carrier system for the cells allowing proliferation and differentiation in response to the local biological signalling cues. A dodecylic amide of HA named HYADD3®, (11% of carboxyl group substitution, Mw=165kDa) was investigated. HYADD3® solutions having concentration of 20, 40, 60 mg/ml were tested for rheological characterization. Different solvents were used: bidistilled filtered (0.22 mm filter) water and a 0.15 M NaCl water solution.A suspension of the stem cells was obtained and mixed with the gels to give a cell concentration of 1x106 cells/ml. Proliferation of cells within the gels was determined using the alamarBlueTM assay (Life Technologies). The alamarBlueTM assay showed that stem cells seeded within the HYADD3® were able to proliferate and remained viable up to 14 days. Thus indicating that the gel had good diffusion properties allowing nutrient supply and gaseous exchange to the cells. The results of viscoelastic study showed the ability to design an injectable material able to mimic the viscoelastic behavior of NP. Moreover, the HYADD3® gel shows suitable properties for the incorporation of stem cells for use in tissue engineering (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Dep. Experimental and Clinical Medicine, University of Magna Graecia, Germaneto Catanzaro, Italy Institute of Composite and Biomedical Materials, CNR and CRIB, University of Naples “Federico II”, Naples, Italy Biomaterials & Biomimetics Group, King’s College London, Guy’s Campus, , London SE1 9RT, UK Fidia Advanced Biopolymers, 35031 Abano Terme, Italy (literal)

Titolo

• Engineering a stem cell loaded injectable nucleus pulposus substitute (literal)

Prodotto di

• Biomateriali ed ingegneria dei tessuti (PM.P02.004.002) (Modulo)
• Institute for composite and biomedical materials (IMCB) (Istituto)

Autore CNR

• LUIGI AMBROSIO (Unità di personale interno)
• ASSUNTA BORZACCHIELLO (Unità di personale interno)
• Antonio Gloria (Unità di personale esterno)

Insieme di parole chiave

• Parole chiave di "Engineering a stem cell loaded injectable nucleus pulposus substitute" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ASSUNTA BORZACCHIELLO (Unità di personale interno)
• LUIGI AMBROSIO (Unità di personale interno)
• Antonio Gloria (Unità di personale esterno)

Prodotto

• Institute for composite and biomedical materials (IMCB) (Istituto)
• Biomateriali ed ingegneria dei tessuti (PM.P02.004.002) (Modulo)

Insieme di parole chiave di

• Parole chiave di "Engineering a stem cell loaded injectable nucleus pulposus substitute" (Insieme di parole chiave)