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Purification, cloning and characterization of XendoU, a novel endoribonuclease involved in processing of intron-encoded small nucleolar RNAs in Xenopus laevis (Articolo in rivista)

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Purification, cloning and characterization of XendoU, a novel endoribonuclease involved in processing of intron-encoded small nucleolar RNAs in Xenopus laevis (Articolo in rivista) (literal)

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Laneve P., Altieri F., Fiori M.E., Scaloni A., Bozzoni I., Caffarelli E. (2003)
Purification, cloning and characterization of XendoU, a novel endoribonuclease involved in processing of intron-encoded small nucleolar RNAs in Xenopus laevis
in The Journal of biological chemistry (Print)
(literal)

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Laneve P., Altieri F., Fiori M.E., Scaloni A., Bozzoni I., Caffarelli E. (literal)

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Despite the pletora of functions played by endoribonucleases in cellular metabolism, only a few enzymes were purified and characterised in higher eukaryotes. The identification of such novel enzyme provides further insights into the molecular mechanisms of the biosynthesis of metazoan snoRNAs, which participate in one of the most important cellular pathway: the ribosome biogenesis. The paper is published in a valued international journal with an impact factor of 6.482. (literal)

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In this paper it is reported the purification, from Xenopus laevis oocyte nuclear extracts, of a new endoribonuclease, XendoU, that is involved in the processing of some intron-encoded box C/D small nucleolar RNAs (snoRNAs) from their host pre-mRNA. Such an activity has never been reported before and has several uncommon features that make it quite a novel enzyme: it is poly(U)-specific, it requires Mn2+ ions, and it produces molecules with 2'-3'-cyclic phosphate termini. (literal)

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1 UNI Sapienza (Istituto Pasteur - Fondazione Cenci-Bolognetti), 2 UNI Sapienza (Dipartimento di Scienze Biochimiche \"A. Rossi Fanelli\"), 3 CNR (Istituto per i Sistemi di Produzione Animale in Ambiente Mediterraneo, Laboratorio di Proteomica e Spettrometria di Massa), 4 CNR (Istituto di Biologia e Patologia Molecolari). (literal)

Titolo

Purification, cloning and characterization of XendoU, a novel endoribonuclease involved in processing of intron-encoded small nucleolar RNAs in Xenopus laevis (literal)

Abstract

Here we report the purification, from Xenopus laevis oocyte nuclear extracts, of a new endoribonuclease, XendoU, that is involved in the processing of the intron-encoded box C/D U16 small nucleolar RNA (snoRNA) from its host pre-mRNA. Such an activity has never been reported before and has several uncommon features that make it quite a novel enzyme: it is poly(U)-specific, it requires Mn2+ ions, and it produces molecules with 2'-3'-cyclic phosphate termini. Even if XendoU cleaves U-stretches, it displays some preferential cleavage on snoRNA precursor molecules. XendoU also participates in the biosynthesis of another intron-encoded snoRNA, U86, which is contained in the NOP56 gene of Xenopus laevis. A common feature of these snoRNAs is that their production is alternative to that of the mRNA, suggesting an important regulatory role for all the factors involved in the processing reaction. (literal)

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