http://www.cnr.it/ontology/cnr/individuo/prodotto/ID11288

Spindle assembly and cytokinesis in the absence of chromosomes during Drosophila male meiosis. (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Spindle assembly and cytokinesis in the absence of chromosomes during Drosophila male meiosis. (Articolo in rivista) (literal)

Anno

2003-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1083/jcb.200211029 (literal)

Alternative label

Bucciarelli E , Giansanti MG , Bonaccorsi S , Gatti M. (2003)
Spindle assembly and cytokinesis in the absence of chromosomes during Drosophila male meiosis.
in The Journal of cell biology
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Bucciarelli E , Giansanti MG , Bonaccorsi S , Gatti M. (literal)

Pagina inizio

993 (literal)

Pagina fine

999 (literal)

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This paper is published in a highly valued journal with an impact factor of 12.52 in 2002. Our demonstration that chromosomes are not always necessary for proper spindle assembly and cell division stimulated a specific commentary, which appeared in the same issue of the journal (Earnshaw and Carmena, JCB, 160: 989-990, 2003). In addition, a figure from our paper was chosen to illustrate the cover of this JCB issue. Finally, our work was highlighted in the Editors Choice of Science (Science, 300: 395-397, 2003) (literal)

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160 (literal)

Rivista

?The ?Journal of cell biology (Rivista)

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M. Gatti a disposizione dell'IBPM (literal)

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7 (literal)

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Our findings show that Drosophila spermatocytes can form a morphologically normal spindle in the absence of chromosomes and thus in the absence of a high concentration of Ran-GTP in the center of the cell. These chromosome-free cells also assemble regular cytokinetic structures and cleave normally. These results are at odds with the classical view that chromosomes play an essential role in spindle formation and dynamics, indicating these processes are controlled by chromosome-independent factors during Drosophila male meiosis. (literal)

Note

ISI Web of Science (WOS) (literal)

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1 UNI Sapienza (Dipartimento Genetica e Biologia Molecolare); 2 CNR (Istituto Biologia e Patologia Molecolari). (literal)

Titolo

Spindle assembly and cytokinesis in the absence of chromosomes during Drosophila male meiosis. (literal)

Abstract

A large body of work indicates that chromosomes play a key role in the assembly of both acentrosomal and centrosome-containing spindles. In animal systems, the absence of chromosomes either prevents spindle formation or allows the assembly of a metaphase-like spindle that fails to evolve into an ana-telophase spindle. Here, we show that Drosophila secondary spermatocytes can assemble morphologically normal spindles in the absence of chromosomes. The Drosophila mutants fusolo and solofuso are severely defective in chromosome segregation and produce secondary spermatocytes that are devoid of chromosomes. The centrosomes of these anucleated cells form robust asters that give rise to bipolar spindles that undergo the same ana-telophase morphological transformations that characterize normal spindles. The cells containing chromosome-free spindles are also able to assemble regular cytokinetic structures and cleave normally. In addition, chromosome-free spindles normally accumulate the Aurora B kinase at their midzones. This suggests that the association of Aurora B with chromosomes is not a prerequisite for its accumulation at the central spindle, or for its function during cytokinesis. (literal)
A large body of work indicates that chromosomes play a key role in the assembly of both a centrosomal and centrosome-containing spindles. In animal systems, the absence of chromosomes either prevents spindle formation or allows the assembly of a metaphase-like spindle that fails to evolve into an ana-telophase spindle. Here, we show that Drosophila secondary spermatocytes can assemble morphologically normal spindles in the absence of chromosomes. The Drosophila mutants fusolo and solofuso are severely defective in chromosome segregation and produce secondary spermatocytes that are devoid of chromosomes. The centrosomes of these anucleated cells form robust asters that give rise to bipolar spindles that undergo the same ana-telophase morphological transformations that characterize normal spindles. The cells containing chromosome-free spindles are also able to assemble regular cytokinetic structures and cleave normally. In addition, chromosome-free spindles normally accumulate the Aurora B kinase at their midzones. This suggests that the association of Aurora B with chromosomes is not a prerequisite for its accumulation at the central spindle, or for its function during cytokinesis. (literal)

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