http://www.cnr.it/ontology/cnr/individuo/prodotto/ID11137

In vitro toxicity of mercuric chloride on rabbit spermatozoa motility and cell membrane integrity (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

In vitro toxicity of mercuric chloride on rabbit spermatozoa motility and cell membrane integrity (Articolo in rivista) (literal)

Anno

2010-01-01T00:00:00+01:00 (literal)

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10.1080/10934521003651598 (literal)

Alternative label

Jana Slivkova; Peter Massanyi; Flavia Pizzi; Jozef Trandzik; Shubhadeep Roychoudhury; Norbert Lukac; Marianna Dankova;Viera Almasiova (2010)
In vitro toxicity of mercuric chloride on rabbit spermatozoa motility and cell membrane integrity
in Journal of Environmental Science and Health, Part A; Taylor and Francis, Abingdon (Regno Unito)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Jana Slivkova; Peter Massanyi; Flavia Pizzi; Jozef Trandzik; Shubhadeep Roychoudhury; Norbert Lukac; Marianna Dankova;Viera Almasiova (literal)

Pagina inizio

767 (literal)

Pagina fine

774 (literal)

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45 (literal)

Rivista

Journal of Environmental Science and Health, Part A (Rivista)

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The Journal of Environmental Science and Health, Part A addresses topics, including, but not limited to, pollutants, xenobiotics and pharmaceuticals in water, soil, sediment and air, and their impact on human health or environmental quality. Impact Factor 2008 5-Year: 1.992 (literal)

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8 (literal)

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Pubblicazione scientifica riguardante l'effetto del mercurio sulla qualità del materiale seminale di coniglio. I dati raccolti confermano un effetto negativo sulla motilità degli spermatozoi di alte concentrazioni di mercurio nel seme (literal)

Note

Scopus (literal)
Google Scholar (literal)
PubMe (literal)
ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Jana Slivkova, Peter Massanyi, Shubhadeep Roychoudhury, Norbert Lukac Department of Animal Physiology, Slovak University of Agriculture, Nitra, Slovak Republic; Flavia Pizzi IBBA CNR Institute of Agricultural Biology and Biotechnology, National Research Council, Milano, Italy; Jozef Trandzik Department of Zoology and Anthropology, Constantine the Philosopher University, Faculty of Natural Sciences, Nitra, Slovak Republic; Viera Almasiova Department of Anatomy, Histology and Physiology, University of Veterinary Medicine, Kosice, Slovak Republic; Marianna Dankova Institute of Histology and Embryology, Pavol Jozef Safarik University, Kosice, Slovak Republic (literal)

Titolo

In vitro toxicity of mercuric chloride on rabbit spermatozoa motility and cell membrane integrity (literal)

Abstract

Mercury as one of heavy metals belongs among the oldest and the best-known toxicants and contaminations of environment. In this study the effects of mercuric chloride on the motility and structural integrity of rabbit spermatozoa in vitro were investigated. The spermatozoa motility was evaluated using CASA method and Annexin analysis was used for detection of structural changes. The concentration of mercury in medium vary from 5.0 to 83.3 ¼g HgCl2/mL. At Time 0 the highest motility in control group was detected (67.09±8.72%). Motility in groups with mercury administration was decreased in comparison with control. Significant differences were detected in groups with 50.0 83.3 ¼g HgCl2/mL (p<0.001). After 60 and 120 minutes of incubation with mercury chloride the motility was significantly decreased in almost all experimental groups. Progressive motility had a decreasing trend in all experimental groups. Significant differences in group 6.25 83.3 ¼g HgCl2/mL at Time 60 and 120 were found. Significant differences were detected in all experimental groups, expect the group with lowest mercury chloride concentration. The concentration decreasing spermatozoa progressive motility to 50% of control was detected for groups 50.0 83.3 at Time 0, for groups 12.5 83.3 at Time 60 and 120, decreasing from 36.46±18.73% to 1.03±2.50%. Detail evaluation of spermatozoa distance (DAP, DCL, and DSL) and velocity (VAP, VCL, and VSL) parameters as well as straightness (STR), linearity (LIN), wobble (WOB), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) of spermatozoa were decreased in groups with the highest mercury concentration in comparison with control group in all time periods. Detection of spermatozoa with disordered membrane using Annexin analysis was implemented for groups with higher mercury concentration and control. Analysis proved higher occurrence of positive spermatozoa in the mercury exposed groups. Few Annexin positive reactions from all spermatozoa were detected in control group. In mercury exposed groups positive reaction proved alteration in anterial part of head (acrosome), connection part (connection piece) and in mitochondrial segment. Detected data evidently confirm adverse effects of high mercury chloride concentrations in rabbit semen on spermatozoa motility parameters. (literal)

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