Mitochondrial DNA and RNA isolation from small amounts of various potato tissues. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Mitochondrial DNA and RNA isolation from small amounts of various potato tissues. (Articolo in rivista) (literal)

Anno

• 2001-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/BF02824080 (literal)

Alternative label

• Scotti N., Cardi T., Maréchal-Drouard L. (2001)
  Mitochondrial DNA and RNA isolation from small amounts of various potato tissues.
  in Plant molecular biology reporter (Dordr., Online)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Scotti N., Cardi T., Maréchal-Drouard L. (literal)

Pagina inizio

• 67a (literal)

Pagina fine

• 67h (literal)

Convegno

• Plant Mol. Biol. Rept. 19: 67a-67h (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 19 (literal)

Rivista

• Plant molecular biology reporter (Rivista)

Note

• Scopus (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Research Institute for Vegetable and Ornamental Plant Breeding, CNR-IMOF, via Universita' 133, 80055, Portici, Italy; Institut de Biologie Moléeculaire des Plantes du CNRS, 12 rue du Géenéeral Zimmer, 67084, Strasbourg Cedex, France (literal)

Titolo

• Mitochondrial DNA and RNA isolation from small amounts of various potato tissues. (literal)

Abstract

• We present a fast and simple protocol for purification of mitochondrial DNA and RNA from small amounts of potato tissue including tubers, leaves, flowers, and flower buds. This method uses a high ionic strength medium to isolate mitochondria and extract mitochondrial DNA and RNA from a single preparation and is easily adaptable to other plant species. The mitochondrial DNA was not contaminated by plastid DNA, was fully restrictable and was successfully used for PCR, cloning and Southern analyses. Similarly, the isolated mitochondrial RNA was not contaminated (flower buds) or only slightly contaminated (leaves) by plastid RNA. RNA prepared according to our method was acceptable for northern and RT-PCR analyses. (literal)

Prodotto di

• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)
• Ottenimento di nuovi prodotti per la salute, l'alimentazione e l'industria mediante interventi genetici e biotecnologici nelle piante (AG.P01.006.001) (Modulo)

Autore CNR

• TEODORO CARDI (Unità di personale interno)
• NUNZIA SCOTTI (Persona)

Incoming links:

Autore CNR di

• TEODORO CARDI (Unità di personale interno)
• NUNZIA SCOTTI (Persona)

Prodotto

• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)
• Ottenimento di nuovi prodotti per la salute, l'alimentazione e l'industria mediante interventi genetici e biotecnologici nelle piante (AG.P01.006.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Plant molecular biology reporter (Rivista)