Localization of stilbene synthase in Vitis vinifera L. during berry development (Articolo in rivista)

Type
Label
  • Localization of stilbene synthase in Vitis vinifera L. during berry development (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/s00709-008-0309-8 (literal)
Alternative label
  • Fornara V.; Onelli E.; Sparvoli F.; Rossoni M.; Aina R.; Marino G.; Citterio S. (2008)
    Localization of stilbene synthase in Vitis vinifera L. during berry development
    in Protoplasma
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Fornara V.; Onelli E.; Sparvoli F.; Rossoni M.; Aina R.; Marino G.; Citterio S. (literal)
Pagina inizio
  • 83 (literal)
Pagina fine
  • 93 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 233 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • DOI 10.1007/s00709-008-0309-8 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Fornara V.; Aina R.; Marino G.; Citterio S. - Dipartimento di Scienze dell'Ambiente e del Territorio, Università degli Studi di Milano-Bicocca Piazza della Scienza n.1 - 20126 Milan, Italy Onelli E. - Dipartimento di Biologia, Università degli Studi di Milano, Via Celoria n.26 - 20133 Milan, Italy. Sparvoli F. - Istituto di Biologia e Biotecnologia Agraria, CNR, Via Bassini n.15 - 20133 Milan, Italy. Rossoni M. - Dipartimento di Produzione vegetale, Università degli Studi di Milano, Via Caloria 2 - 20133 Milan, Italy. (literal)
Titolo
  • Localization of stilbene synthase in Vitis vinifera L. during berry development (literal)
Abstract
  • The localization of stilbene synthase (STS, EC 2.3.1.95 ) in grape berry (Vitis vinifera L.) was investigated during fruit development. The berries were collected at 2, 4, 7, 11 and 15 weeks post-flowering from the cultivar Nebbiolo during the 2005 and 2006 growing seasons. HPLC analysis showed that berries accumulated cis- and trans-isomers of resveratrol mainly in exocarp throughout fruit development. Immunodetection of STS protein was performed on berry extracts and sections using an antibody specifically developed against recombinant grape STS1. In agreement with resveratrol presence, stilbene synthase was found in berry exocarp tissues during all stages of fruit development: the epidermal cells labeled were few and were randomly distributed, whereas nearly all the outer hypodermis cells were STS positive. The STS signal decreased gradually from exocarp to mesocarp, where the protein was detected only occasionally. At the sub-cellular level, STS was found predominantly within vesicles (of varying size), along plasma membrane and in cell wall, suggesting protein secretion in the apoplast compartment. Despite the differences in fruit size and structure, the STS localization was the same before and after veraison, the relatively short development period during which the firm green berries begin to soften and change color. Nevertheless, the amount of protein detected in both exocarp and mesocarp decreased significantly in ripe berries, in agreement with the lower resveratrol content measured in the same tissues. The STS location in exocarp cell wall is consistent with the STS role in synthesizing defense compounds, and supports the already formulated hypothesis that a differential localization of phenylpropanoid biosynthetic machinery regulates the deposition of specific secondary products at different action sites within cells. (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Prodotto
Autore CNR di
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
Insieme di parole chiave di
data.CNR.it