http://www.cnr.it/ontology/cnr/individuo/prodotto/ID10980

Tubulin-based polymorphism (TBP): a new tool, based on functionally relevant sequences, to asses genetic diversity in plant species (Articolo in rivista)

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Tubulin-based polymorphism (TBP): a new tool, based on functionally relevant sequences, to asses genetic diversity in plant species (Articolo in rivista) (literal)

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Bardini M., Lee D , Donini P. , Mariani A., Gianì S. , Toschi M., Lowe C., and Breviario D (2004)
Tubulin-based polymorphism (TBP): a new tool, based on functionally relevant sequences, to asses genetic diversity in plant species
in Genome (Ott., Print); National Research Council Of Canada-Nrc Research Press, Ottawa (Canada)
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Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Bardini M., Lee D , Donini P. , Mariani A., Gianì S. , Toschi M., Lowe C., and Breviario D (literal)

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M. Bardini. Università degli Studi di Milano, Dipartimento di Biologia, Via Celoria 26, Milano, Italy. D.Lee, P.Donini and C. Lowe. National Institute of Agricultural Botany, Huntingdon Road, Cambridge,CB3 OLE, UK. A. Mariani Istituto di Ricerche sul Miglioramento Genetico delle piante Foraggere, Via della Madonna Alta 130 Perugia, Italy. M. Toschi, S. Gianì and D. Breviario Istituto Biologia e Biotecnologia agraria-CNR, Via Bassini 15 Milano, Italy (literal)

Titolo

Tubulin-based polymorphism (TBP): a new tool, based on functionally relevant sequences, to asses genetic diversity in plant species (literal)

Abstract

TBP (tubulin-based polymorphism) is a new molecular marker based tool that relies on the presence of intron-specific DNA polymorphisms of the plant beta-tubulin gene family. The multifunctional and essential role of the tubulin proteins is reflected in the conservation of regions within their primary amino acid sequence. The ubiquitous nature of this gene family can be exploited using primers that amplify the first intron of different beta-tubulin isotypes, revealing specific fingerprints. The method is rapid, simple, and reliable and does not require preliminary sequence information of the plant genome of interest. The ability of TBP to discriminate between accessions and species in oilseed rape, coffee, and lotus is shown. In all cases, TBP was able to detect specific genetic polymorphisms in the context of a simplified and readily appreciable pattern of DNA amplification. The application of TBP for assessing genetic diversity and genome origins in disseminated plant landraces rather than in highly inbred cultivated species is also discussed. (literal)

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