Saporin and ricin A chain follow different intracellular routes to enter the cytosol of intoxicated cells. (Articolo in rivista)

Type
Label
  • Saporin and ricin A chain follow different intracellular routes to enter the cytosol of intoxicated cells. (Articolo in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1111/j.1742-4658.2005.04908.x (literal)
Alternative label
  • Vago R., Marsden C.J. , Lord J.M., Ippoliti R., Flavell D.J., Flavell S.-U , Ceriotti A., Fabbrini M.S. (2005)
    Saporin and ricin A chain follow different intracellular routes to enter the cytosol of intoxicated cells.
    in The FEBS journal (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Vago R., Marsden C.J. , Lord J.M., Ippoliti R., Flavell D.J., Flavell S.-U , Ceriotti A., Fabbrini M.S. (literal)
Pagina inizio
  • 4983 (literal)
Pagina fine
  • 4995 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Le conoscenze acquisite permetteranno di ottimizzare il rilascio nelle cellule tumorali di chimere costruite a partire da saporina (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 272 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • La rivista su cui l'articolo è stato pubblicato è nata nel 2005 dalla rivista scientifica di chimica European Journal of Biochemistry con fattore di impatto 3,843 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Pubblicazione scientifica che riguarda il percorso intracellulare compiuto da una tossina di pianta (saporina) appartenente alla famiglia delle RIP, la cui capostipite e’ Ricina nell’ intossicare le cellule di mammifero (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • VR, MSF: Dibit-S Raffaele Scientific Institute, Milan, Italy MCJ, LJM: Department of Biological Sciences, University of Warwick, Coventry, UK IR: Dipartimento di Biologia di Base ed Applicata, Universita` degli Studi di L’Aquila, Italy FDJ, FS-U: The Simon Flavell Leukaemia Research Unit, University Department of Pathology, Southampton, UK CA: Istituto di Biologia e Biotecnologia Agraria, CNR, Milan, Italy. (literal)
Titolo
  • Saporin and ricin A chain follow different intracellular routes to enter the cytosol of intoxicated cells. (literal)
Abstract
  • Several protein toxins, such as the potent plant toxin ricin, enter mammalian cells by endocytosis and undergo retrograde transport via the Golgi complex to reach the endoplasmic reticulum (ER). In this compartment the catalytic moieties exploit the ER-associated degradation (ERAD) pathway to reach their cytosolic targets. Bacterial toxins such as cholera toxin or Pseudomonas exotoxin A carry KDEL or KDEL-like C-terminal tetrapeptides for efficient delivery to the ER. Chimeric toxins containing monomeric plant ribosome-inactivating proteins linked to various targeting moieties are highly cytotoxic, but it remains unclear how these molecules travel within the target cell to reach cytosolic ribosomes. We investigated the intracellular pathways of saporin, a monomeric plant ribosome-inactivating protein that can enter cells by receptor-mediated endocytosis. Saporin toxicity was not affected by treatment with Brefeldin A or chloroquine, indicating that this toxin follows a Golgi-independent pathway to the cytosol and does not require a low pH for membrane translocation. In intoxicated Vero or HeLa cells, ricin but not saporin could be clearly visualized in the Golgi complex using immunofluorescence. The saporin signal was not evident in the Golgi, but was found to partially overlap with that of a late endosome D lysosome marker. Consistently, the toxicities of saporin or saporin-based targeted chimeric polypeptides were not enhanced by the addition of ER retrieval sequences. Thus, the intracellular movement of saporin differs from that followed by ricin and other protein toxins that rely on Golgi-mediated retrograde transport to reach their retrotranslocation site. (literal)
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