Development of a novel GFP-based ratiometric excitation and emission pH indicator for intracellular studies (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Development of a novel GFP-based ratiometric excitation and emission pH indicator for intracellular studies (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1529/biophysj.105.074708 (literal)

Alternative label

• Bizzarri, R; Arcangeli, C; Arosio, D; Ricci, F; Faraci, P; Cardarelli, F; Beltram, F (2006)
  Development of a novel GFP-based ratiometric excitation and emission pH indicator for intracellular studies
  in Biophysical journal (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Bizzarri, R; Arcangeli, C; Arosio, D; Ricci, F; Faraci, P; Cardarelli, F; Beltram, F (literal)

Pagina inizio

• 3300 (literal)

Pagina fine

• 3314 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=1&SID=N26jMdn66DjbpIo2B7p&page=3&doc=29 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 90 (literal)

Rivista

• Biophysical journal (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 9 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Scuola Normale Super Pisa, Natl Enterprise Nanosci & Nanotechnol NEST, CNR, INFM, I-56126 Pisa, Italy (literal)

Titolo

• Development of a novel GFP-based ratiometric excitation and emission pH indicator for intracellular studies (literal)

Abstract

• We report on the development of the F64L/S65T/T203Y/L231H GFP mutant ( E 2 GFP) as an effective ratiometric pH indicator for intracellular studies. E 2 GFP shows two distinct spectral forms that are convertible upon pH changes both in excitation and in emission with pK close to 7.0. The excitation of the protein at 488 and 458 nm represents the best choice in terms of signal dynamic range and ratiometric deviation from the thermodynamic pK. This makes E-2 GFP ideally suited for imaging setups equipped with the most widespread light sources and filter settings. We used E(2)GFP to determine the average intracellular pH (pH(i)) and spatial pH(i) maps in two different cell lines, CHO and U-2 OS, under physiological conditions. In CHO, we monitored the evolution of the pH(i) during mitosis. We also showed the possibility to target specific subcellular compartments such as nucleoli ( by fusing E(2)GFP with the transactivator protein of HIV, ( Tat) and nuclear promyelocytic leukemia bodies ( by coexpression of promyelocytic leukemia protein). (literal)

Prodotto di

• Proteine fluorescenti per il bioimaging avanzato (MD.P06.022.001) (Modulo)

Autore CNR

• FABIO BELTRAM (Unità di personale esterno)
• DANIELE AROSIO (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Development of a novel GFP-based ratiometric excitation and emission pH indicator for intracellular studies" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• DANIELE AROSIO (Unità di personale interno)
• FABIO BELTRAM (Unità di personale esterno)

Prodotto

• Proteine fluorescenti per il bioimaging avanzato (MD.P06.022.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biophysical journal (Rivista)

Insieme di parole chiave di

• Parole chiave di "Development of a novel GFP-based ratiometric excitation and emission pH indicator for intracellular studies" (Insieme di parole chiave)