http://www.cnr.it/ontology/cnr/individuo/prodotto/ID10534

Mesenchymal stem cells impair in vivo T-cell priming by dendritic cells (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Mesenchymal stem cells impair in vivo T-cell priming by dendritic cells (Articolo in rivista) (literal)

Anno

2011-01-01T00:00:00+01:00 (literal)

Alternative label

Chiesa S. 1,2,3, Morbelli S. 4, Morando S.1,2, Massollo M. 4, Marini C. 5, Bertoni A. 1,2, Frassoni F. 6, Tabera Bartolomé S. 7,8, Sambucetid G. 4, Traggiai E. 3,9, Uccelli A. 1,2,10 (2011)
Mesenchymal stem cells impair in vivo T-cell priming by dendritic cells
in Proceedings of the National Academy of Sciences of the United States of America
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Chiesa S. 1,2,3, Morbelli S. 4, Morando S.1,2, Massollo M. 4, Marini C. 5, Bertoni A. 1,2, Frassoni F. 6, Tabera Bartolomé S. 7,8, Sambucetid G. 4, Traggiai E. 3,9, Uccelli A. 1,2,10 (literal)

Pagina inizio

17384 (literal)

Pagina fine

17389 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

108 (literal)

Rivista

Proceedings of the National Academy of Sciences of the United States of America (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

Pubblicato on-line: 29 Settembre 2011. Corresponding author: S. Chiesa (sabrina.chiesa@ospedale-gaslini.ge.it.) IF 2010: 9.771 (literal)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

1. Department of Neurosciences, Ophthalmology and Genetics, University of Genova. 2. Advanced Biotechnology Center, Genova. 3. 2nd Division of Pediatrics, Laboratory of Immunology and Rheumatic Diseases, IRCCS, Institute G. Gaslini Hospital, Genova. 4. Department of Nuclear Medicine, University of Genova. 5. CNR-IBFM, Genova Section, Genova. 6. Stem Cell Centre and Cellular Therapy, San Martino Hospital, Genova. 7. Institute of Neuroscience of Castilla y León, University of Salamanca, 37007 Salamanca, Spain; 8. Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y Leon, University of Salamanca, Salamanca (Spagna) 9. Novartis Institute for Biomedical Research, Translational Medicine, Basel (Svizzera) 10. Center of Excellence for Biomedical Research, University of Genova. (literal)

Titolo

Mesenchymal stem cells impair in vivo T-cell priming by dendritic cells (literal)

Abstract

Dendritic cells (DC) are highly specialized antigen-presenting cells characterized by the ability to prime T-cell responses. Mesenchymal stem cells (MSC) are adult stromal progenitor cells displaying immunomodulatory activities including inhibition of DC maturation in vitro. However, the specific impact of MSC on DC functions, upon in vivo administration, has never been elucidated. Here we show that murine MSC impair Toll-like receptor-4 induced activation of DC resulting in the inhibition of cytokines secretion, down-regulation of molecules involved in the migration to the lymph nodes, antigen presentation to CD4+ T cells, and cross-presentation to CD8+ T cells. These effects are associated with the inhibition of phosphorylation of intracellular mitogen-activated protein kinases. Intravenous administration of MSC decreased the number of CCR7 and CD49d²1 expressing CFSE-labeled DC in the draining lymph nodes and hindered local antigen priming of DO11.10 ovalbumin-specific CD4+ T cells. Upon labeling of DC with technetium-99m hexamethylpropylene amine oxime to followtheir in vivo biodistribution, we demonstrated that intravenous injection of MSC blocks, almost instantaneously, the migration of subcutaneously administered ovalbumin-pulsed DC to the draining lymph nodes. These findings indicate thatMSC significantly affect DC ability to prime T cells in vivo because of their inability to home to the draining lymph nodes and further confirmMSC potentiality as therapy for immune-mediated diseases. (literal)

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