REGULATION AND POSSIBLE FUNCTION OF THE ENDOCANNABINOID SYSTEM IN MYOGENESIS (Comunicazione a convegno)

Type
Label
  • REGULATION AND POSSIBLE FUNCTION OF THE ENDOCANNABINOID SYSTEM IN MYOGENESIS (Comunicazione a convegno) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Alternative label
  • Cristoforo Silvestri, Fabiana Piscitelli, Andrea Martella and Vincenzo Di Marzo (2010)
    REGULATION AND POSSIBLE FUNCTION OF THE ENDOCANNABINOID SYSTEM IN MYOGENESIS
    in ICRS 20th Symposium, Lund, Sweden
    (literal)
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  • Cristoforo Silvestri, Fabiana Piscitelli, Andrea Martella and Vincenzo Di Marzo (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Skeletal muscle arises from stem cells originating in the embryonic mesoderm. These same stem cells are the source of satellite cells, which are associated with skeletal muscle and are crucial to muscle growth and regeneration. The pluripotent murine myoblast cell line C2C12 is often utilized as a model of skeletal muscle differentiation and regeneration due to its ability to undergo myogenesis. Upon withdrawal from the cell cycle, C2C12 cells initiate a muscle specific gene programme resulting in cellular fusion and formation of multinucleated myotubes. Recent studies have identified the expression of cannabinoid receptors (Cnr) in skeletal muscle and the role of the endocannabinoid system (ECS) as a regulator of insulin sensitivity, oxidation and glucose metabolism. The role of the ECS in muscle cells differentiation remains to be determined. Utilizing C2C12 cells, quantitative PCR and isotope-dilution LC-MS, we analysed the expression of various components of the ECS during myogenesis, and obtained preliminary results on its possible role in skeletal muscle differentiation. We report that C2C12cells express Cnr1, and much less, if any, Cnr2, and show that Cnr1 expression increases drastically during the early stages of myogenesis and then decreases.Interestingly, expression of 2-arachidonoylglycerol (2-AG) anabolic and catabolic enzymes, Dagla and Mgll, decreases and increases, respectively, during myogenesis. Analysis of 2-AG levels in differentiating C2C12 cells showed that, consistent with the changes observed in Dagla and Mgll expression, its levels decrease significantly with myogenesis. Incubation of C2C12 cells during the first 4 days of differentiation with 2-AG or its catabolite arachidonic acid (AA) resulted in significantly lower levels of the differentiated muscle marker Myh1. Observation of differentiated C2C12 cells did not reveal any obvious morphological differences between 2-AG- or AA-treated cells. In contrast to this, treatment with anandamide did not result in consistent changes in Myh1 expression in differentiating C2C12 cells; however it did result in the formation of thickened and branched myotubes, indicating an increase in cell fusion and/or protein synthesis, which affect myotube size. These data show that the ECS is tightly regulated during the process of myogenesis and represent the first evidence of the emerging role that the ECS may play in muscle differentiation and/or regeneration, with likely different functions exerted by 2-AG and AEA. (literal)
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  • Endocannabinoid Research Group, Istituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, Via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy (literal)
Titolo
  • REGULATION AND POSSIBLE FUNCTION OF THE ENDOCANNABINOID SYSTEM IN MYOGENESIS (literal)
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