LIVER AND ADIPOSE TISSUE ENDOCANNABINOID TONE IN DIET-INDUCEDOBESITY IS DEPENDENT ON APOLIPOPROTEIN E EXPRESSION ANDASSOCIATED WITH HEPATIC STEATOSIS AND INSULIN RESISTANCE (Comunicazione a convegno)

Type
Label
  • LIVER AND ADIPOSE TISSUE ENDOCANNABINOID TONE IN DIET-INDUCEDOBESITY IS DEPENDENT ON APOLIPOPROTEIN E EXPRESSION ANDASSOCIATED WITH HEPATIC STEATOSIS AND INSULIN RESISTANCE (Comunicazione a convegno) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Alternative label
  • Vincenzo Di Marzo, Alexander Bartelt, Concetta Mele,Pierangelo Orlando, Stefania Petrosino, Alessia Ligresti,Klaus Toedter, Ludger Scheja and Joerg Heeren (2010)
    LIVER AND ADIPOSE TISSUE ENDOCANNABINOID TONE IN DIET-INDUCEDOBESITY IS DEPENDENT ON APOLIPOPROTEIN E EXPRESSION ANDASSOCIATED WITH HEPATIC STEATOSIS AND INSULIN RESISTANCE
    in ICRS 20th Symposium, Lund, Sweden
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Vincenzo Di Marzo, Alexander Bartelt, Concetta Mele,Pierangelo Orlando, Stefania Petrosino, Alessia Ligresti,Klaus Toedter, Ludger Scheja and Joerg Heeren (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • The levels of endocannabinoids and/or CB1 receptors are elevated in the liver and epididymal white adipose tissue (WAT), and reduced in the subcutaneous WAT, of mice with high fat diet (HFD) induced obesity. Furthermore, in obese rodents and humans, 2- arachidonoylglycerol levels are associated with fatty liver, high amounts of visceral adipose tissue, inflammatory markers and insulin resistance. On the other hand, apolipoprotein E (ApoE) deficiency is associated with non-alcoholic steatohepatitis and reduced fat accumulation in the WAT. Here we investigated, in apoE-/- and wild-type (WT) mice, the effect of a HFD on: 1) subcutaneous and epididymal WAT accumulation, liver triglycerides, inflammatory markers in the WAT and liver, and insulin resistance, and 2) WAT and hepatic fatty acid composition, endocannabinoid levels and CB1 receptor and endocannabinoid metabolic enzyme mRNA expression. After a 16 week HFD, both WT and ApoE-/- mice exhibited higher amounts of WAT and liver triglycerides and impaired insulin resistance. However, ApoE-/- mice showed lower WAT accumulation and body weight, less fasting leptin, glucose and insulin levels, and more hepatic steatosis than WT mice. In glucose and insulin clearance experiments, the areas under the curve for both insulin and glucose after HFD were higher in WT than ApoE-/- mice, which also exhibited higher expression of inflammatory markers (TNFa, MCP-1, CD68, Emr1) in the liver, but lower in the epididymal WAT. The typical HFD-induced elevation of endocannabinoid in the liver or epididymal WAT was significantly higher or lower, respectively, in ApoE-/- than WT mice, whereas the HFD-induced decrease of endocannabinoid tissue and CB1 mRNA expression in the subcutaneous WAT was absent in the transgenic mice. These differential alterations in endocannabinoid levels reflected changes in the expression/activity of endocannabinoid catabolic enzymes in the WAT, or the availability of esterified arachidonic acid (the ultimate endocannabinoid biosynthetic precursor) in the liver. In conclusion, we report that dysfunctional endocannabinoid tone in the liver and WAT of mice with diet-induced obesity is dependent on the presence of ApoE and is associated with hepatic steatosis, inflammation in the WAT and liver, and insulin resistance. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1,3Endocannabinoid Research Group, 1Istituto di Chimica Biomolecolare and 3Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Via Campi Flegrei 34, Comprensorio Olivetti, 80078 Pozzuoli (NA), Italy; 2Department of Biochemistry and Molecular Biology II: Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Martinistraße 52, 20246 Hamburg, Germany (literal)
Titolo
  • LIVER AND ADIPOSE TISSUE ENDOCANNABINOID TONE IN DIET-INDUCEDOBESITY IS DEPENDENT ON APOLIPOPROTEIN E EXPRESSION ANDASSOCIATED WITH HEPATIC STEATOSIS AND INSULIN RESISTANCE (literal)
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