15-deoxy-delta 12,14-prostaglandin J(2) inhibits the synthesis of the acute phase protein SIP24 in cartilage: Involvement of COX-2 in resolution of inflammation. (Articolo in rivista)

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  • 15-deoxy-delta 12,14-prostaglandin J(2) inhibits the synthesis of the acute phase protein SIP24 in cartilage: Involvement of COX-2 in resolution of inflammation. (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Alternative label
  • Ulivi V. 1,2, Cancedda R. 1,2, Descalzi Cancedda F. 1,3 (2008)
    15-deoxy-delta 12,14-prostaglandin J(2) inhibits the synthesis of the acute phase protein SIP24 in cartilage: Involvement of COX-2 in resolution of inflammation.
    in Journal of cellular physiology (Print)
    (literal)
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  • Ulivi V. 1,2, Cancedda R. 1,2, Descalzi Cancedda F. 1,3 (literal)
Pagina inizio
  • 433 (literal)
Pagina fine
  • 441 (literal)
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  • 217 (literal)
Rivista
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  • Pubblicato on-line: 9 Luglio 2008. Corresponding author: F. Descalzi (fiorella.descalzi@istge.it). I.F.: 4.313 (literal)
Note
  • ISI Web of Science (WOS) (literal)
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  • 1. Istituto Nazionale per la Ricerca sul Cancro, Genova 2. Dipartimento di Oncologia, Biologia e Genetica, Università degli Studi, Genova 3. Istituto Bioimmagini e Fisiologia Molecolare, Consiglio Nazionale delle Ricerche, Sezione di Genova, Genova (literal)
Titolo
  • 15-deoxy-delta 12,14-prostaglandin J(2) inhibits the synthesis of the acute phase protein SIP24 in cartilage: Involvement of COX-2 in resolution of inflammation. (literal)
Abstract
  • We previously demonstrated that, in the MC615 cartilage cell line, the p38/NF-kB pathway is activated both during differentiation and in response to an inflammatory stimulus. In both cases, the p38/NF-kB pathway activation leads to the expression of the lipocalin SIP24 and of COX-2. Given the fact that, in the same cells, the COX-2 expression is sustained during the inflammation resolution, at the same time that the SIP24 expression is suppressed, in the present study we tested the hypothesis that COX-2 products play a role in SIP24 repression. Taken together, our results suggest that, during the resolution of inflammation, COX-2 represses the acute phase protein SIP24 and restores physiological conditions, possibly through a pathway involving PPARgamma. Experimental evidences being the following: (1) 15-deoxy-delta 12,14-prostaglandin J(2), but not PGE(2): (i) inhibits the expression of SIP24 in the inflammatory phase and induces COX-2 synthesis; (ii) represses NF-kB activation induced by LPS; (iii) represses the synthesis of microsomal PGE Synthase-1 induced by LPS. (2) PPARgamma and PPARalpha are present in MC615 cells in both proliferating and hyperconfluent cultures. (3) PPARgamma ligand GW7845, but not PPARalpha ligand GW7647: (i) represses the expression of SIP24 induced by LPS; (ii) induces COX-2 expression. (4) p38 is involved in the PPARgamma mediated induction of COX-2. In fact 15-deoxy-delta 12,14-prostaglandin J(2) activates p38 and the cell pretreatment with the p38 specific inhibitor SB203580 represses the expression of COX-2 induced by both the 15-deoxy-delta12,14-prostaglandin J(2) and the PPARgamma ligand GW7845. (literal)
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