Screening of Italian rice cultivars for the expression of MYB and WRKY genes and genes involved in the ROS signalling pathway following BTH treatment (Abstract/Poster in atti di convegno)

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  • Screening of Italian rice cultivars for the expression of MYB and WRKY genes and genes involved in the ROS signalling pathway following BTH treatment (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Alternative label
  • Baldoni E., Mattana M., Locatelli F., Picchi V., Consonni R., Genga A. (2011)
    Screening of Italian rice cultivars for the expression of MYB and WRKY genes and genes involved in the ROS signalling pathway following BTH treatment
    in 10th International Conference on Reactive Oxygen and Nitrogen Species in plants, Budapest, 5-8 luglio 2011
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Baldoni E., Mattana M., Locatelli F., Picchi V., Consonni R., Genga A. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • 5-8 luglio 2011 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Italy is the largest rice (Oryza sativa L.) producer and exporter in Europe, with more than 50% of the total paddy production. Even if rice growth on the most productive irrigated lands in the world has reached almost the maximum potential production, the achievement of the optimum yield is made difficult by environmental stresses, such as water deficiency, soil salinity and pathogen attack. In particular, rice blast, caused by the fungus Magnaporthe oryzae, is the most severe disease of rice worldwide. The development of new rice varieties with a higher resistance to blast is of great interest also in our country. The molecular response to biotic stresses has been largely studied on rice model cultivars, such as Nipponbare, whereas little is known on Italian varieties. The aim of our research is to provide a characterization of Italian rice cultivars for the expression of genes coding for transcription factors (TF) and genes involved in the reactive oxygen species (ROS) signalling pathway mediating disease resistance in plants. Benzothiadiazole (BTH), a functional analog of Salicylic acid (SA), is one of the so-called plant activators that protect various plants from infectious diseases. BTH treatments have been used by several authors to study disease plant response, since it is a potent inducers of both SAR and PR genes1. Moreover, some works demonstrated that BTH can induce oxidative burst and hypersensitive cell death2. Plants of 12 rice Italian cultivars were grown in greenhouse in soil for 20 days and BTH was sprayed onto leaves. The cultivars have been selected on the basis of their resistance/susceptibility to blast3. Expression analysis through qRT-PCR was first performed on the WRKY45 gene, known to be induced by BTH4, in order to verify the occurrence of the BTH response in treated plants. Among seven TF encoding genes analyzed for the expression level, one WRKY and two myb genes seemed to be differentially expressed in resistant and susceptible cultivars. The qRT-PCR data revealed that the expression level of these genes increased in most of the resistant cultivars and decreased or did not change in the susceptible ones, thus suggesting a positive correlation between their expression level and the resistance phenotype of the rice cultivars. One of these myb genes is involved in the ROS signalling network mediating disease resistance (literal)
Note
  • Poster (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Baldoni E., Mattana M., Locatelli F., Genga A.: CNR-IBBA Milano; Consonni R.: CNR-ISMAC Milano (literal)
Titolo
  • Screening of Italian rice cultivars for the expression of MYB and WRKY genes and genes involved in the ROS signalling pathway following BTH treatment (literal)
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