Screening of italian rice cultivars for the expression of myb and WRKY genes under abiotic and biotic stresses. (Abstract/Poster in atti di convegno)

Type

• Prodotto della ricerca (Classe)
• Abstract/Poster in atti di convegno (Classe)

Label

• Screening of italian rice cultivars for the expression of myb and WRKY genes under abiotic and biotic stresses. (Abstract/Poster in atti di convegno) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Alternative label

• Baldoni E., Mattana M., Locatelli F., Consonni R., Genga A. (2010)
  Screening of italian rice cultivars for the expression of myb and WRKY genes under abiotic and biotic stresses.
  in 54° Convegno Socetà Italiana di Genetica Agraria, Matera, 27-30 settembre 2010
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Baldoni E., Mattana M., Locatelli F., Consonni R., Genga A. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• 27-30 Settembre (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Italy is the largest rice (Oryza sativa L.) producer and exporter in Europe, with more than 50% of the total paddy production. Even if rice growth on the most productive irrigated lands in the world has reached almost the maximum potential production, the achievement of the optimum yield is made difficult by environmental stresses, such as water deficiency, soil salinity and pathogen attack. The development of new rice varieties with a higher tolerance/resistance to both abiotic and biotic stresses is of great interest also in our country, for the adaptation of rice to suboptimal climate and soil conditions. The molecular response to abiotic and biotic stresses has been largely studied on rice model cultivars, such as Nipponbare, whereas little is known on Italian varieties. The aim of our research is to provide a characterization of Italian rice cultivars for the expression of genes coding for transcription factors involved in the response to water stress and pathogen attack. Osmotic stress:PEG-mediated osmotic stress was imposed on hydroponically grown plantlets of 12 Italian cultivars, selected on the basis of their tolerance/sensitivity to water stress. The stress response was evaluated through measurement of physiological parameters (RWC, ion leakage or chlorophyll fluorescence). qRT-PCR expression analysis was performed on leaves and roots collected from plants grown under normal and stress conditions. The expression of the OsDREB2A gene, known to be induced by drought, was evaluated in order to verify the occurrence of stress response in treated plants. The expression analysis of 10 myb and three WRKY genes, known to be involved in drought response, is in progress on the selected cultivars. BTH-induced stress: Benzothiadiazole (BTH), a functional analog of Salicylic acid (SA), is one of the so-called plant activators that protect various plants from infectious diseases. BTH treatments have been used by several authors to study disease plant response. Plants of 12 rice Italian cultivars were grown in greenhouse in soil for 20 days and BTH was sprayed onto leaves. The cultivars have been selected on the basis of their resistance/susceptibility to blast. Expression analysis through qRT-PCR was first performed on the WRKY45 gene, known to be induced by BTH, in order to verify the occurrence of the BTH response in treated plants. Among seven genes analyzed for the expression level, one WRKY and two myb genes seemed to be differentially expressed in resistant and susceptible cultivars. The qRT-PCR data revealed that the expression level of these genes increased in most of the resistant cultivars and decreased or did not change in the susceptible ones, thus suggesting a positive correlation between their expression level and the resistance phenotype of the rice cultivars. (literal)

Note

• Poster (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Consonni R.: CNR-ISMAC Milano; Baldoni E.,Mattana M., Locatelli F., Genga A. : CNR-IBBA Milano (literal)

Titolo

• Screening of italian rice cultivars for the expression of myb and WRKY genes under abiotic and biotic stresses. (literal)

Prodotto di

• Studio di metaboliti e matrici naturali mediante NMR e loro utilizzo e funzione nel settore agroalimentare, merceologico e della salute umana (PM.P06.018.001) (Modulo)
• Istituto per lo studio delle macromolecole (ISMAC) (Istituto)
• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Biotecnologie agro-industriali per il miglioramento genetico, il rispetto dell'ambiente e la tutela dei prodotti, la produzione di proteine e metaboliti di interesse alimentare, salutistico e farmacologico. (AG.P01.004.001) (Modulo)

Autore CNR

• ELENA BALDONI (Persona)
• MONICA MATTANA (Persona)
• ANNAMARIA GENGA (Unità di personale interno)
• ROBERTO CONSONNI (Unità di personale interno)
• FRANCA LOCATELLI (Persona)

Insieme di parole chiave

• Parole chiave di "Screening of italian rice cultivars for the expression of myb and WRKY genes under abiotic and biotic stresses." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Istituto per lo studio delle macromolecole (ISMAC) (Istituto)
• Biotecnologie agro-industriali per il miglioramento genetico, il rispetto dell'ambiente e la tutela dei prodotti, la produzione di proteine e metaboliti di interesse alimentare, salutistico e farmacologico. (AG.P01.004.001) (Modulo)
• Studio di metaboliti e matrici naturali mediante NMR e loro utilizzo e funzione nel settore agroalimentare, merceologico e della salute umana (PM.P06.018.001) (Modulo)

Autore CNR di

• ROBERTO CONSONNI (Unità di personale interno)
• FRANCA LOCATELLI (Persona)
• MONICA MATTANA (Persona)
• ANNAMARIA GENGA (Unità di personale interno)
• ELENA BALDONI (Persona)

Insieme di parole chiave di

• Parole chiave di "Screening of italian rice cultivars for the expression of myb and WRKY genes under abiotic and biotic stresses." (Insieme di parole chiave)