Sorting and turnover of tonoplast proteins: the Arabidopsis potassium channel TPK1 as a model (Comunicazione a convegno)

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  • Sorting and turnover of tonoplast proteins: the Arabidopsis potassium channel TPK1 as a model (Comunicazione a convegno) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
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  • Maîtrejean M., Wudick M.M., Voelker C., Pedrazzini E., Czempinski K., Vitale A. (2010)
    Sorting and turnover of tonoplast proteins: the Arabidopsis potassium channel TPK1 as a model
    in II° Congresso della Società Italiana di Biologia Vegetale, Roma
    (literal)
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  • Maîtrejean M., Wudick M.M., Voelker C., Pedrazzini E., Czempinski K., Vitale A. (literal)
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  • Comunicazione orale su invito, scelta dagli organizzatori fra i poster (literal)
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  • 12-14/07/2010 (literal)
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  • We are using the potassium channel AtTPK1 as a model to study how integral tonoplast proteins are sorted to their destination and eventually degraded. Starting from a TPK1-GFP fusion, domains were substituted by the corresponding ones of the plasma membrane homologue AtTPK4. Expression in Arabidopsis protoplasts indicated that the cytosolic C-terminus of AtTPK1 is necessary and sufficient for tonoplast sorting of the potassium channel. TPK1-GFP formed dimers both in transgenic Arabidopsis and in protoplasts, whereas a number of TPK1/TPK4 fusions that remained located in the endoplasmic reticulum (ER) were unable to assemble correctly and interacted extensively with the ER chaperone BiP. This indicated that chimeric fusions remained in the ER because of conformational defects leading to retention by ER quality control. Pulse-chase labelling of protoplasts indicated that TPK1-GFP has a half life time of at least 24 hours and that its slow turnover involves internalization into the vacuole, leading to the vacuolar accumulation of soluble, released GFP that was originally fused to the C-terminus. Supported by the EU Marie Curie RTN (MRTN-CT-2006–035833). (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • MM, EP, AV: IBBA-CNR; MMW, CV, KC: University of Potsdam (Germany) (literal)
Titolo
  • Sorting and turnover of tonoplast proteins: the Arabidopsis potassium channel TPK1 as a model (literal)
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