http://www.cnr.it/ontology/cnr/individuo/prodotto/ID102508
Quality trait assessment of conventional and transgenic lettuce (Lactuca sativa L.) at harvesting by NMR-metabolic profiling (Abstract/Poster in atti di convegno)
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- Quality trait assessment of conventional and transgenic lettuce (Lactuca sativa L.) at harvesting by NMR-metabolic profiling (Abstract/Poster in atti di convegno) (literal)
- Anno
- 2009-01-01T00:00:00+01:00 (literal)
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Testone G., Sobolev A.P., Nicolodi C., Capitani D., Santoro F., Iannelli M.A., Brosio E., Mannina L., Giannino D. (2009)
Quality trait assessment of conventional and transgenic lettuce (Lactuca sativa L.) at harvesting by NMR-metabolic profiling
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- Testone G., Sobolev A.P., Nicolodi C., Capitani D., Santoro F., Iannelli M.A., Brosio E., Mannina L., Giannino D. (literal)
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- The changes of hydrophilic and lipophilic constituents in leaves of transgenic and conventional lettuce (Lactuca sativa L. cultivar 'Cortina') plants were investigated. NMR metabolic profiles [of three transgenic lines (T3B12, T7B7, T7B14) overexpressing the E. coli asparagine synthetase A gene were compared with those of the wild type counterparts at 21, 56 and 64 days after sowing (DAS). A set of hydrophilic metabolites, including amino acids, significantly discriminated transgenic from wild type lines, even if the effect of leaf physiological maturation is predominant. The metabolic patterns were used to picture the nutritional traits of head leaves at 56DAS, suitable for the harvest in a indoor spring cycle. The T3B12 and T7B7 individuals shared increased contents of amino acids compared to controls, whilst T7B14 plants skewed from the other two, showing a peculiar raise of leaf inulin. In T3B12 and T7B7 leaves, endogenous asparagine synthase genes were up-regulated and the nitrate content diverged from that of controls, suggesting that the pMAC:asnA transgene globally impacted on the enhancement of leaf nitrogen status. In T7B14 plants, key genes of inulin biosynthesis were triggered (fructose-fructosyl
and sucrose-sucrosyl transferases), whilst those of catabolism were down-regulated (fructan hydrolase and transferase), in concomitance with inulin accumulation. (literal)
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- Quality trait assessment of conventional and transgenic lettuce (Lactuca sativa L.) at harvesting by NMR-metabolic profiling (literal)
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