Multivariate analysis of gene expression and metabolic content changes to decipher rice response to blast (Abstract/Poster in atti di convegno)

Type
Label
  • Multivariate analysis of gene expression and metabolic content changes to decipher rice response to blast (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Alternative label
  • Abbruscato P., Fumagalli E., Faivre-Rampant O., Baldoni E., Genga A., Piffanelli P., Consonni R. (2008)
    Multivariate analysis of gene expression and metabolic content changes to decipher rice response to blast
    in Convegno: 7th Plant GEM (Plant Genomics European Meeting), Albena (Bulgaria)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Abbruscato P., Fumagalli E., Faivre-Rampant O., Baldoni E., Genga A., Piffanelli P., Consonni R. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • 24-27/09/2008 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Abstract poster: The Ascomycete Magnaporthe grisea is the causal agent of rice (Oryza sativa L.) blast disease, which is considered to be the most important rice disease worldwide. In fact, it is widely distributed and it can be very destructive with yield losses ranging from 10 to 50%, depending upon environmental conditions. To prevent infections, it is normal practice the use of cultivars with race-specific resistance to Magnaporthe grisea and the application of fungicides. An alternative solution is to understand the mechanisms which underlie broad spectrum resistance to host and nonhost M.grisea strains, to develop rice cultivars with durable resistance. In the post-genomic era, deciphering plant responses to stimuli is achieved combining the study of gene expression changes and the subsequent metabolic effects. The metabolome, defined as the total collection of small molecular weight compounds, is the final downstream genome product, participating in reactions required for plant growth, metabolism and defence reactions. Both abiotic stresses (salt, temperature, drought) and pathogen attacks lead to qualitative and quantitative modifications of primary and secondary metabolites. Therefore, detection and identification of metabolite changes are instrumental to understand plant adaptation to stress conditions. In particular, tracking down metabolic pathways activated by host and nonhost strains is a novel approach towards the understanding of the durable resistance to M. grisea. The main objective of our study is to integrate information collected from both metabolic and gene profiling in rice following blast infection to gain insights on cellular responses to host and nonhost strains of Magnaporthe grisea. NMR techniques were used to evaluate the metabolic changes in Nipponbare leaves of plantlets challenged with host and nonhost strains, at different times after infection (24 and 48 hours post-inoculation). In addition, gene expression analyses of WRKYs transcriptional factors and defence genes were carried out on the same leaf samples to identify specific genes involved in response to M. grisea infection. (literal)
Note
  • Poster (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • BE, GA: Istituto di Biologia e Biotecnologia Agraria, CNR, Milano FE, CR: Istituto per lo Studio delle Macromolecole, CNR, Milano AP, PP: Parco Tecnologico Padano, Lodi F-RO: Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier, France (literal)
Titolo
  • Multivariate analysis of gene expression and metabolic content changes to decipher rice response to blast (literal)
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