Comparison of protein expression in human deltoideus and vastus lateralis muscles using two-dimensional gel electrophoresis. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Comparison of protein expression in human deltoideus and vastus lateralis muscles using two-dimensional gel electrophoresis. (Articolo in rivista) (literal)

Anno

• 2005-01-01T00:00:00+01:00 (literal)

Alternative label

• Capitanio D. 1, Vigano A. 1,3, Ricci E. 2, Cerretelli P. 3, Wait R. 4, Gelfi C. 1 (2005)
  Comparison of protein expression in human deltoideus and vastus lateralis muscles using two-dimensional gel electrophoresis.
  in Proteomics (Weinh., Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Capitanio D. 1, Vigano A. 1,3, Ricci E. 2, Cerretelli P. 3, Wait R. 4, Gelfi C. 1 (literal)

Pagina inizio

• 2577 (literal)

Pagina fine

• 2586 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 5 (literal)

Rivista

• Proteomics (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Institute of Molecular Bioimaging and Physiology, CNR, Segrate (MI), Italy 2. Department of Neurology, Policlinico Gemelli, Catholic University, Rome, Italy 3. Department of Sciences and Biomedical Technologies, University of Milan, Milan, Italy 4. Faculty of Medicine, Kennedy Institute of Rheumatology, Imperial College London, London, UK (literal)

Titolo

• Comparison of protein expression in human deltoideus and vastus lateralis muscles using two-dimensional gel electrophoresis. (literal)

Abstract

• We have used two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) to study the expression of contractile and regulatory proteins in human vastus lateralis and deltoideus muscles, in order to understand protein turnover and isoform switching in muscles with the same fiber-type composition but different functional properties. We demonstrate a two- to six-fold overexpression of enzymes associated with glycolysis, the tricarboxylic acid cycle, oxidative phosphorylation, and substrate transport in vastus lateralis compared to deltoideus. Expression levels of contractile protein isoforms correlated to the proportion of slow-twitch fibers in deltoideus compared to vastus lateralis are consistent with the different contractile properties of the two muscles. Two proteins involved in free radical homeostasis were differentially expressed, suggesting a direct relationship between radical scavenging and the muscle function. The application of 2-DE and MS to studies of muscle physiology thus offers a more comprehensive assessment of the molecular determinants of muscle function than traditional approaches. (literal)

Prodotto di

• Genomica e proteomica per la ricerca di biomarcatori in malattie neurodegenerative e nei tumori (ME.P05.002.001) (Modulo)
• Institute of molecular bioimaging and physiology (IBFM) (Istituto)

Autore CNR

• CECILIA GELFI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Comparison of protein expression in human deltoideus and vastus lateralis muscles using two-dimensional gel electrophoresis." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• CECILIA GELFI (Unità di personale interno)

Prodotto

• Institute of molecular bioimaging and physiology (IBFM) (Istituto)
• Genomica e proteomica per la ricerca di biomarcatori in malattie neurodegenerative e nei tumori (ME.P05.002.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Proteomics (Rivista)

Insieme di parole chiave di

• Parole chiave di "Comparison of protein expression in human deltoideus and vastus lateralis muscles using two-dimensional gel electrophoresis." (Insieme di parole chiave)