On the molecular structure of human neuroserpin polymers (Articolo in rivista)

Type
Label
  • On the molecular structure of human neuroserpin polymers (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/prot.23197 (literal)
Alternative label
  • Santangelo MG; Noto R; Levantino M; Cupane A; Ricagno S, Pezzullo M, Bolognesi M, Mangione MR, Martorana V, Manno M (2012)
    On the molecular structure of human neuroserpin polymers
    in Proteins (Print); WILEY-BLACKWELL, MALDEN 02148,MA (Stati Uniti d'America)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Santangelo MG; Noto R; Levantino M; Cupane A; Ricagno S, Pezzullo M, Bolognesi M, Mangione MR, Martorana V, Manno M (literal)
Pagina inizio
  • 8 (literal)
Pagina fine
  • 13 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://onlinelibrary.wiley.com/doi/10.1002/prot.23197/abstract?systemMessage=Wiley+Online+Library+will+be+disrupted+on+17+December+from+at+23%3A00+GMT+%2818%3A00+EST%29+for+essential+maintenance+for+approximately+one+hour (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 80 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 1 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Natl Res Council Italy, Inst Biophys, I-90146 Palermo, Italy; Univ Palermo, Dept Phys, I-90123 Palermo, Italy; Univ Milan, Inst Biophys CNR, Dept Biomol Sci & Biotechnol, Milan, Italy; Univ Milan, CIMAINA, Milan, Italy (literal)
Titolo
  • On the molecular structure of human neuroserpin polymers (literal)
Abstract
  • The polymerization of serpins is at the root of a large class of diseases; the molecular structure of serpin polymers has been recently debated. In this work, we study the polymerization kinetics of human neuroserpin by Fourier Transform Infra Red spectroscopy and by time-lapse Size Exclusion Chromatography. First, we show that two distinct neuroserpin polymers, formed at 45 and 85°C, display the same isosbestic points in the Amide I2 band, and therefore share common secondary structure features. We also find a concentration independent polymerization rate at 45°C suggesting that the polymerization rate-limiting step is the formation of an activated monomeric species. The polymer structures are consistent with a model that predicts the bare insertion of portions of the reactive center loop into the A ²-sheet of neighboring serpin molecule, although with different extents at 45 and 85°C (literal)
Editore
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Autore CNR di
Prodotto
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
Editore di
Insieme di parole chiave di
data.CNR.it