Long-range and long-term interferometric tracking by static and dynamic force-clamp optical tweezers (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Long-range and long-term interferometric tracking by static and dynamic force-clamp optical tweezers (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1364/OE.19.022364 (literal)

Alternative label

• A. Guiggiani; B. Torre; A. Contestabile; F. Benfenati; M. Basso; M. Vassalli; F. Difato (2011)
  Long-range and long-term interferometric tracking by static and dynamic force-clamp optical tweezers
  in Optics express
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• A. Guiggiani; B. Torre; A. Contestabile; F. Benfenati; M. Basso; M. Vassalli; F. Difato (literal)

Pagina inizio

• 22364 (literal)

Pagina fine

• 22376 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 23 (literal)

Rivista

• Optics express (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Università di Firenze, Dipartimento di Sistemi e Informatica, Florence, Italy Italian Institute of Technology (IIT), Dept. of Nanophysics, Genoa, Italy Italian Institute of Technology (IIT), Dept. of Neuroscience and Brain Technologies, Genoa, Italy National Research Council of Italy, Institute of biophysics, Genoa, Italy (literal)

Titolo

• Long-range and long-term interferometric tracking by static and dynamic force-clamp optical tweezers (literal)

Abstract

• Optical tweezers are recognized single-molecule technique to resolve forces and motion on the molecular scale. Complex biological phenomena, such as cell differentiation and locomotion, require long range tracking capabilities with nanometer resolution over an extended period, to resolve molecular processes on the cellular scale. Here we introduce a real-time control of the microscope stage position to perform long-term tracking, with sub-millisecond resolution, of a bead attached to a neuron, preserving sub-nanometer sensitivity on a spatial range of centimeters, seven orders of magnitude larger. Moreover, the suitability of the system is tested by time- modulating the force-clamp condition to study the role of statically and dynamically applied forces in neuronal differentiation (literal)
• Optical tweezers are recognized single-molecule technique to resolve forces and motion on the molecular scale. Complex biological phenomena, such as cell differentiation and locomotion, require long range tracking capabilities with nanometer resolution over an extended period, to resolve molecular processes on the cellular scale. Here we introduce a real-time control of the microscope stage position to perform long-term tracking, with sub-millisecond resolution, of a bead attached to a neuron, preserving sub-nanometer sensitivity on a spatial range of centimeters, seven orders of magnitude larger. Moreover, the suitability of the system is tested by time- modulating the force-clamp condition to study the role of statically and dynamically applied forces in neuronal differentiation. (literal)

Prodotto di

• Processi di membrana nella comunicazione intra- ed inter-cellulare (MD.P01.001.001) (Modulo)
• Istituto di biofisica (IBF) (Istituto)

Autore CNR

• MASSIMO VASSALLI (Unità di personale interno)

Incoming links:

Prodotto

• Istituto di biofisica (IBF) (Istituto)
• Processi di membrana nella comunicazione intra- ed inter-cellulare (MD.P01.001.001) (Modulo)

Autore CNR di

• MASSIMO VASSALLI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Optics express (Rivista)