Activation of telomerase through VEGF signaling promotes neo-angiogenesis (Comunicazione a convegno)

Type
Label
  • Activation of telomerase through VEGF signaling promotes neo-angiogenesis (Comunicazione a convegno) (literal)
Anno
  • 2003-01-01T00:00:00+01:00 (literal)
Alternative label
  • L. Della Pietra*, G. Zaccagnini#, C. Gaetano#, S. Nanni*, A. Grasselli*, A. Mangoni#, R. Benvenuto#, A. Germani#, F. Moretti*°, A. Pontecorvi*§, A. Sacchi*, S. Bacchetti*, M. C. Capogrossi# and A. Farsetti*°. (2003)
    Activation of telomerase through VEGF signaling promotes neo-angiogenesis
    in Convegno Annuale " Struttura e funzione del genoma", Cortona, 11-13 Aprile 2003
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • L. Della Pietra*, G. Zaccagnini#, C. Gaetano#, S. Nanni*, A. Grasselli*, A. Mangoni#, R. Benvenuto#, A. Germani#, F. Moretti*°, A. Pontecorvi*§, A. Sacchi*, S. Bacchetti*, M. C. Capogrossi# and A. Farsetti*°. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Abstract Book online (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • *Mol. Oncogenesis Lab. IRE-CRS, #Lab. Vascul. Pathol., IDI, §Inst. Med. Pat., Catholic Univ., °Inst. Neurobiol. & Molecular Medicine, CNR, Rome, Italy. (literal)
Titolo
  • Activation of telomerase through VEGF signaling promotes neo-angiogenesis (literal)
Abstract
  • In mammals, age-dependent telomere dysfunction may contribute to a reduction in cell viability, altered differentiation functions and impaired regenerative/proliferative responses. Recent advances indicate that sustained level of telomerase activity in endothelial cells and precursors may confer a pro-angiogenic phenotype to these cells. We have investigated whether te catalytic subunit of telomerase contributes in vivo to the process of tissue regeneration following hind-limb ischemia and adenoviral-mediated VEGF165 treatment in young and aged rats. Unilateral hind-limb ischemia was induced by surgical dissection of the femoral artery in young (3 months) and aged (22 months) male Fisher rats. Coincidentally, animals were injected i.m. with saline solution, AdNull or AdVEGF165 (5X107 pfu). Evaluation of angiogenesis by capillary count and of telomerase by RT-PCR, immunoistochemistry and TRAP assays were performed on adductor and quadriceps muscles at 3, 8 and 14 days upon treatment (n= ? 6 animals/point). VEGF delivery to ischemic tissues induces angiogenesis and a significant increase of TERTmRNA, TERT protein and telomerase activity in skeletal muscles, vascular endothelial, smooth muscle and satellite skeletal muscle cells in young and, to a lower extent, older animals, presumably as a consequence of tissue remodeling. To explore the molecular mechanisms underlying this phenomenon, VEGF-dependent induction of TERT expression and activity was reproduced in vitro in differentiated murine myoblasts C2C12. Such increase was abrogated by addition of 7-Nitroindazole, a Nitric Oxide Synthases inhibitor, indicating that VEGF-mediated synthesis of nitric oxide is involved in this process. In addition, adenoviral-mediated hTERT gene transfer in young rats induced angiogenesis 3 and 8 days after ischemia with an efficiency similar to that elicited by VEGF165, as assessed by measurement of capillaries density. Thus TERT appears to directly contribute to angiogenesis in vivo, suggesting an extracurricular telomerase function. (literal)
Prodotto di
Autore CNR

Incoming links:


Autore CNR di
Prodotto
data.CNR.it