Virus-derived small RNAs in tomato plants infected by Tomato yellow leaf curl Sardinia virus analysed by deep sequencing (Abstract/Comunicazione in atti di convegno)

Type

• Prodotto della ricerca (Classe)
• Abstract/Comunicazione in atti di convegno (Classe)

Label

• Virus-derived small RNAs in tomato plants infected by Tomato yellow leaf curl Sardinia virus analysed by deep sequencing (Abstract/Comunicazione in atti di convegno) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Alternative label

• MIOZZI L., PANTALEO V., NORIS E., BURGYAN J. & ACCOTTO G.P. (2011)
  Virus-derived small RNAs in tomato plants infected by Tomato yellow leaf curl Sardinia virus analysed by deep sequencing
  in SIV 2011, Orvieto, September, 11-14, 2011
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• MIOZZI L., PANTALEO V., NORIS E., BURGYAN J. & ACCOTTO G.P. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• oral communication (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• INTERVENTO ORALE (literal)

Note

• Comunicazione (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• ML; PV; NE; BJ; AGP: IVV CNR (literal)

Titolo

• Virus-derived small RNAs in tomato plants infected by Tomato yellow leaf curl Sardinia virus analysed by deep sequencing (literal)

Abstract

• Background: The begomovirus Tomato yellow leaf curl Sardinia virus (TYLCSV) induces a devastating disease of tomato crops in the Mediterranean region. TYLCSV has a 2.7 kb circular single-stranded DNA genome that includes a bidirectional promoter driving the synthesis of viral RNA transcripts. These are recognised by the plant RNA silencing machinery as foreign RNAs that generate viral small interfering RNAs (v-siRNAs). Methods: A cDNA library of small RNAs was obtained from TYLCSV-infected tomato plants and sequenced on Solexa/Illumina platform. The subset of v-siRNAs was identified and characterized. Results: TYLCSV-derived siRNAs, mainly of 21-22 nt, spanned the entire viral genome but were discontinuously distributed throughout it. Viral-siRNAs from the non-coding region were poorly represented; the most abundant v-siRNAs were from the coat protein and the Rep/C4 genes and were mainly of sense polarity. The major hot spots were experimentally validated by reverse oligo dot blot analysis. Conclusions: The first high-resolution v-siRNA map for TYLCSV is reported. The mechanism/s of v-siRNA biogenesis and their role in antiviral plant response are discussed. (literal)

Prodotto di

• Resistenze convenzionali e non-convenzionali a importanti virosi e fitoplasmosi di specie coltivate (AG.P01.014.001) (Modulo)

Autore CNR

• EMANUELA NORIS (Unità di personale interno)
• JOZSEF BURGYAN (Persona)
• VITANTONIO PANTALEO (Persona)
• LAURA MIOZZI (Unità di personale interno)
• GIAN PAOLO ACCOTTO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Virus-derived small RNAs in tomato plants infected by Tomato yellow leaf curl Sardinia virus analysed by deep sequencing" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• GIAN PAOLO ACCOTTO (Unità di personale interno)
• EMANUELA NORIS (Unità di personale interno)
• VITANTONIO PANTALEO (Persona)
• LAURA MIOZZI (Unità di personale interno)
• JOZSEF BURGYAN (Persona)

Prodotto

• Resistenze convenzionali e non-convenzionali a importanti virosi e fitoplasmosi di specie coltivate (AG.P01.014.001) (Modulo)

Insieme di parole chiave di

• Keywords of "Virus-derived small RNAs in tomato plants infected by Tomato yellow leaf curl Sardinia virus analysed by deep sequencing" (Insieme di parole chiave)