Irreversible gelation of thermally unfolded lysozyme: structural and mechanical properties of lysozyme aggregates (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Irreversible gelation of thermally unfolded lysozyme: structural and mechanical properties of lysozyme aggregates (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s00249-009-0503-4 (literal)

Alternative label

• Raccosta S.; Manno M. (1); Bulone D. (1); Giacomazza D. (1); Militello V.; Martorana V. (1); San Biagio P. L. (1) (2010)
  Irreversible gelation of thermally unfolded lysozyme: structural and mechanical properties of lysozyme aggregates
  in European biophysics journal
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Raccosta S.; Manno M. (1); Bulone D. (1); Giacomazza D. (1); Militello V.; Martorana V. (1); San Biagio P. L. (1) (literal)

Pagina inizio

• 1007 (literal)

Pagina fine

• 1017 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 39 (literal)

Rivista

• European biophysics journal (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 6 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1) IBF Palermo (literal)

Titolo

• Irreversible gelation of thermally unfolded lysozyme: structural and mechanical properties of lysozyme aggregates (literal)

Abstract

• The formation of protein aggregates is important in many fields of life science and technology. The morphological and mechanical properties of protein solutions depend upon the molecular conformation and thermodynamic and environmental conditions. Non-native or unfolded proteins may be kinetically trapped into irreversible aggregates and undergo precipitation or gelation. Here, we study the thermal aggregation of lysozyme in neutral solutions. We characterise the irreversible unfolding of lysozyme by differential scanning calorimetry. The structural properties of aggregates and their mechanisms of formation with the eventual gelation are studied at high temperature by spectroscopic, rheological and scattering techniques. The experiments show that irreversible micron-sized aggregates are organised into larger clusters according to a classical mechanism of diffusion and coagulation, which leads to a percolative transition at high concentrations. At a smaller length scale, optical and atomic force microscopy images reveal the existence of compact aggregates, which are the origin of the aggregation irreversibility. (literal)

Prodotto di

• Processi di aggregazione biomolecolare (MD.P01.002.001) (Modulo)
• Istituto di biofisica (IBF) (Istituto)

Autore CNR

• DANIELA GIACOMAZZA (Persona)
• MAURO MANNO (Unità di personale interno)
• DONATELLA BULONE (Unità di personale interno)
• PIER LUIGI SAN BIAGIO (Unità di personale interno)
• VINCENZO MARTORANA (Unità di personale interno)

Incoming links:

Autore CNR di

• MAURO MANNO (Unità di personale interno)
• DANIELA GIACOMAZZA (Persona)
• PIER LUIGI SAN BIAGIO (Unità di personale interno)
• VINCENZO MARTORANA (Unità di personale interno)
• DONATELLA BULONE (Unità di personale interno)

Prodotto

• Istituto di biofisica (IBF) (Istituto)
• Processi di aggregazione biomolecolare (MD.P01.002.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• European biophysics journal (Rivista)