http://www.cnr.it/ontology/cnr/individuo/prodotto/ID9788

Chlorella Viruses Prevent Multiple Infections by Depolarizing the Host Membrane. (Articolo in rivista)

Type

Label

Chlorella Viruses Prevent Multiple Infections by Depolarizing the Host Membrane. (Articolo in rivista) (literal)

Anno

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

Alternative label

Greiner, T., Frohns, F., Kang, M., Van Etten, J., Käsmann, A., Moroni, A., Hertel, B., and Thiel, G. (2009)
Chlorella Viruses Prevent Multiple Infections by Depolarizing the Host Membrane.
in Journal of general virology (Print)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Greiner, T., Frohns, F., Kang, M., Van Etten, J., Käsmann, A., Moroni, A., Hertel, B., and Thiel, G. (literal)

Pagina inizio

Pagina fine

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

Rivista

Note

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Greiner, T., Frohns, F., Käsmann, A., Hertel, B., and Thiel, G.: Tech Univ Darmstadt, Inst Bot, D-64287 Darmstadt, Germany Kang, M., Van Etten, J.: Univ Nebraska, Dept Plant Pathol and Nebraska Ctr Virol, Lincoln, NE 68583 USA Moroni, A.: Dip. di Biologia Università di Milano e CNR-IBF Sezione di Milano Via Celoria,26 20133 Milano Italy (literal)

Titolo

Chlorella Viruses Prevent Multiple Infections by Depolarizing the Host Membrane. (literal)

Abstract

Previous experiments established that when the unicellular green alga Chlorella NC64A is inoculated with two viruses, usually only one virus replicates in a single cell. That is, the viruses mutually exclude one another. In the current study, we explore the possibility that virus-induced host membrane depolarization, at least partially caused by a virus-encoded K(+) channel (Kcv), is involved in this mutual exclusion. Two chlorella viruses, PBCV-1 and NY-2A, were chosen for the study because (i) they can be distinguished by real-time PCR and (ii) they exhibit differential sensitivity to Cs(+), a well-known K(+) channel blocker. PBCV-1-induced host membrane depolarization, Kcv channel activity and plaque formation are only slightly affected by Cs(+), whereas all three NY-2A-induced events are strongly inhibited by Cs(+). The addition of one virus 5-15 min before the other results primarily in replication of the first virus. However, if virus NY-2A-induced membrane depolarization of the host is blocked by Cs(+), PBCV-1 is not excluded. We conclude that virus-induced membrane depolarization is at least partially responsible for the exclusion phenomenon. (literal)

Prodotto di