Fast and slow gating are inherent properties of the K+ channel pore module. (Articolo in rivista)

Type
Label
  • Fast and slow gating are inherent properties of the K+ channel pore module. (Articolo in rivista) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • Abenavoli, A., Di Francesco, M.L., Schroeder, I., Epimashko, S.; Gazzarrini, S., Hansen, U.P., Thiel, G., and Moroni, A. (2009)
    Fast and slow gating are inherent properties of the K+ channel pore module.
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Abenavoli, A., Di Francesco, M.L., Schroeder, I., Epimashko, S.; Gazzarrini, S., Hansen, U.P., Thiel, G., and Moroni, A. (literal)
Pagina inizio
  • 219 (literal)
Pagina fine
  • 229 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 134 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Titolo
  • Fast and slow gating are inherent properties of the K+ channel pore module. (literal)
Abstract
  • Kcv from the chlorella virus PBCV-1 is a viral protein that forms a tetrameric, functional K+ channel in heterologous systems. Kcv can serve as a model system to study and manipulate basic properties of the K+ channel pore because its minimalistic structure (94 amino acids) produces basic features of ion channels, such as selectivity, gating, and sensitivity to blockers. We present a characterization of Kcv properties at the single-channel level. In symmetric 100 mM K+, single-channel conductance is 114+/-11 pS. Two different voltage-dependent mechanisms are responsible for the gating of Kcv. \"Fast\" gating, analyzed by beta distributions, is responsible for the negative slope conductance in the single-channel current-voltage curve at extreme potentials, like in MaxiK potassium channels, and can be explained by depletion-aggravated instability of the filter region. The presence of a \"slow\" gating is revealed by the very low (in the order of 1-4%) mean open probability that is voltage dependent and underlies the time-dependent component of the macroscopic current. (literal)
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