ATP binding to the C-terminus of the Arabidopsis thaliana, AtCLCa, regulates nitrate transport into plant vacuoles. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• ATP binding to the C-terminus of the Arabidopsis thaliana, AtCLCa, regulates nitrate transport into plant vacuoles. (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1074/jbc.M109.005132 (literal)

Alternative label

• De Angeli A.; Moran O.; Wege S.; Filleur S.; Ephritikhine G.; Thomine S.; Barbier-Brygoo H.; Gambale F. (2009)
  ATP binding to the C-terminus of the Arabidopsis thaliana, AtCLCa, regulates nitrate transport into plant vacuoles.
  in The Journal of biological chemistry (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• De Angeli A.; Moran O.; Wege S.; Filleur S.; Ephritikhine G.; Thomine S.; Barbier-Brygoo H.; Gambale F. (literal)

Pagina inizio

• 26526 (literal)

Pagina fine

• 26532 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.jbc.org/content/284/39/26526 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 284 (literal)

Rivista

• ?The ?Journal of biological chemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 39 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Via De Marini 6, 16149 Genova, Italy Institut des Sciences du Végétal, CNRS, 1 Avenue de la Terrasse, 91198 Gif-Sur-Yvette Cedex, France Université Paris 7-Denis Diderot, UFR Sciences du Vivant, 35 rue Hélène Brion, 75205 Paris Cedex 13, France (literal)

Titolo

• ATP binding to the C-terminus of the Arabidopsis thaliana, AtCLCa, regulates nitrate transport into plant vacuoles. (literal)

Abstract

• Nitrate, one of the major nitrogen sources for plants, is stored in the vacuole. Nitrate accumulation within the vacuole is primarily mediated by the NO(3)(-)/H(+) exchanger AtCLCa, which belongs to the chloride channel (CLC) family. Crystallography analysis of hCLC5 suggested that the C-terminal domain, composed by two cystathionine beta-synthetase motifs in all eukaryotic members of the CLC family is able to interact with ATP. However, interaction of nucleotides with a functional CLC protein has not been unambiguously demonstrated. Here we show that ATP reversibly inhibits AtCLCa by interacting with the C-terminal domain. Applying the patch clamp technique to isolated Arabidopsis thaliana vacuoles, we demonstrate that ATP reduces AtCLCa activity with a maximum inhibition of 60%. ATP inhibition of nitrate influx into the vacuole at cytosolic physiological nitrate concentrations suggests that ATP modulation is physiologically relevant. ADP and AMP do not decrease the AtCLCa transport activity; nonetheless, AMP (but not ADP) competes with ATP, preventing inhibition. A molecular model of the C terminus of AtCLCa was built by homology to hCLC5 C terminus. The model predicted the effects of mutations of the ATP binding site on the interaction energy between ATP and AtCLCa that were further confirmed by functional expression of site-directed mutated AtCLCa. (literal)

Prodotto di

• Istituto di biofisica (IBF) (Istituto)
• Meccanismi molecolari della permeabilità di membrana (MD.P01.009.001) (Modulo)
• Processi di membrana nella comunicazione intra- ed inter-cellulare (MD.P01.001.001) (Modulo)

Autore CNR

• OSCAR SANTIAGO MORAN ALBONICO GASPAROTTO (Unità di personale interno)
• ALEXIS DE ANGELI (Persona)
• FRANCO GAMBALE (Persona)

Insieme di parole chiave

• Keywords of "ATP binding to the C-terminus of the Arabidopsis thaliana, AtCLCa, regulates nitrate transport into plant vacuoles." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• FRANCO GAMBALE (Persona)
• OSCAR SANTIAGO MORAN ALBONICO GASPAROTTO (Unità di personale interno)
• ALEXIS DE ANGELI (Persona)

Prodotto

• Istituto di biofisica (IBF) (Istituto)
• Meccanismi molecolari della permeabilità di membrana (MD.P01.009.001) (Modulo)
• Processi di membrana nella comunicazione intra- ed inter-cellulare (MD.P01.001.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• ?The ?Journal of biological chemistry (Rivista)

Insieme di parole chiave di

• Keywords of "ATP binding to the C-terminus of the Arabidopsis thaliana, AtCLCa, regulates nitrate transport into plant vacuoles." (Insieme di parole chiave)