The influence of cholesterol and cone-shaped lipids in Staphylococcus aureus gamma-hemolysins pore formation. (Articolo in rivista)

Type
Label
  • The influence of cholesterol and cone-shaped lipids in Staphylococcus aureus gamma-hemolysins pore formation. (Articolo in rivista) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/s00232-008-9140-6 (literal)
Alternative label
  • Potrich C., H.Bastiani, D.A.Colin, S.Huck, G.Prevost, and M.Dalla Serra. (2009)
    The influence of cholesterol and cone-shaped lipids in Staphylococcus aureus gamma-hemolysins pore formation.
    in The journal of membrane biology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Potrich C., H.Bastiani, D.A.Colin, S.Huck, G.Prevost, and M.Dalla Serra. (literal)
Pagina inizio
  • 13 (literal)
Pagina fine
  • 24 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 227 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • FBK-CNR Istituto di Biofisica, via alla Cascata 56/C, 38100 Povo (Trento), Italy Institut de Bacte┬┤riologie, UPRES EA-3432, 3, rue Koeberle┬┤, 67000 Strasbourg, France (literal)
Titolo
  • The influence of cholesterol and cone-shaped lipids in Staphylococcus aureus gamma-hemolysins pore formation. (literal)
Abstract
  • The natural target of Staphylococcus aureus bicomponent gamma-hemolysins are leucocyte cell membranes. Because a proteinaceous receptor has not been found yet, we checked for the importance of the different membrane lipid compositions by measuring the activity of the toxin on several pure lipid model membranes. We investigated the effect of membrane thickness, fluidity, and presence of nonbilayer lipids and found that the toxin pore-forming ability increased in the presence of phosphocholines with short saturated acyl chains or with unsaturated chains even though not short. An increase of activity was also evident in the presence of cone-shaped lipids like phosphatidylethanolamine or diphytanoylphosphatidylcholine, whereas cylindrical lipids, like sphingomyelin, did not favor the activity. All these results suggest that gamma-hemolysins could bind to the bilayer only if the phosphatidylcholine (PC) head is freely accessible. This condition is satisfied by the concurrent presence of cholesterol and certain lipids, as highlighted by the so-called umbrella model (J. Huang and G. W. Feigenson, Biophys J 76:2142-2157, 1999). According to this model, cholesterol could help to a better exposition of PC head groups only if acyl chains are short or unsaturated. In fact, phosphatidylcholines with more than 13 carbon atoms acyl chains can cover cholesterol molecules; in this way, PC head groups pack tightly, rendering them inaccessible to the toxin, which thus shows a reduced pore-forming ability. (literal)
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