Thioflavin T hydroxylation at basic pH and its effect on amyloid fibril detection. (Articolo in rivista)

Type
Label
  • Thioflavin T hydroxylation at basic pH and its effect on amyloid fibril detection. (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Alternative label
  • Foderà, V., M. Groenning, V. Vetri, F. Librizzi, S. Spagnolo, C. Cornett, L. Oltzen, M. Van de Weert, and M. Leone. (2008)
    Thioflavin T hydroxylation at basic pH and its effect on amyloid fibril detection.
    in The journal of physical chemistry. B
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Foderà, V., M. Groenning, V. Vetri, F. Librizzi, S. Spagnolo, C. Cornett, L. Oltzen, M. Van de Weert, and M. Leone. (literal)
Pagina inizio
  • 15174 (literal)
Pagina fine
  • 15181 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 112 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Univ Palermo, Dipartimento Sci Fis & Astron, I-90123 Palermo, Italy; CNR, Inst Biofis, I-90146 Palermo, Italy; Univ Copenhagen, Dept Pharmaceut & Analyt Chem, Fac Pharmaceut Sci, DK-2100 Copenhagen, Denmark; Univ Copenhagen, Dept Med Chem, Fac Pharmaceut Sci, DK-2100 Copenhagen O, Denmark (literal)
Titolo
  • Thioflavin T hydroxylation at basic pH and its effect on amyloid fibril detection. (literal)
Abstract
  • The fluorescent dye thioflavin T (ThT) is commonly used for in situ amyloid fibril detection. In this work, we focused on the spectroscopic properties and chemical stability of ThT in aqueous solution as a function of pH, temperature, and dye concentration. A reversible hydroxylation process occurs in alkaline solutions, which was characterized using a combination of UV-vis absorption spectroscopy, proton NMR, and density functional theory (DFT). On the basis of these studies, we propose a chemical structure for the hydroxylated form. Finally, by means of fluorescence spectroscopy, ThT hydroxylation effects on in situ amyloid detection have been investigated, providing new insights on the efficiency of the ThT assay for quantitative fibril evaluation at basic pH. (literal)
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