http://www.cnr.it/ontology/cnr/individuo/prodotto/ID9688

P2X(7) presynaptic receptors in adult eat cerebrocortical nerve terminals: a role in ATP-induced glutamate release. (Articolo in rivista)

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P2X(7) presynaptic receptors in adult eat cerebrocortical nerve terminals: a role in ATP-induced glutamate release. (Articolo in rivista) (literal)

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Alternative label

Marcoli M(a,b); Cervetto C(a); Paluzzi P(a); Guarnieri S(a); Alloisio S(c); Thellung S(d); Nobile M(c); Maura G(a,b) (2008)
P2X(7) presynaptic receptors in adult eat cerebrocortical nerve terminals: a role in ATP-induced glutamate release.
in Journal of neurochemistry
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Marcoli M(a,b); Cervetto C(a); Paluzzi P(a); Guarnieri S(a); Alloisio S(c); Thellung S(d); Nobile M(c); Maura G(a,b) (literal)

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a Department of Experimental Medicine, University of Genoa, Genoa, Italy ?b Center of Excellence for Biomedical Research, University of Genoa, Genoa, Italy c ?Institute of Biophysics, CNR, Genoa, Italy d Department of Oncology, Biology and Genetics, University of Genoa, Genoa, Italy (literal)

Titolo

P2X(7) presynaptic receptors in adult eat cerebrocortical nerve terminals: a role in ATP-induced glutamate release. (literal)

Abstract

Although growing evidence suggests that extracellular ATP might play roles in the control of astrocyte/neuron crosstalk in the CNS by acting on P2X(7) receptors, it is still unclear whether neuronal functions can be attributed to P2X(7) receptors. In the present paper, we investigate the location, pharmacological profile, and function of P2X(7) receptors on cerebrocortical nerve terminals freshly prepared from adult rats, by measuring glutamate release and calcium accumulation. The preparation chosen (purified synaptosomes) ensures negligible contamination of non-neuronal cells and allows exposure of 'nude' release-regulating pre-synaptic receptors. To confirm the results obtained, we also carried out specific experiments on human embryonic kidney 293 cells which had been stably transfected with rat P2X(7) receptors. Together, our findings suggest that (i) P2X(7) receptors are present in a subpopulation of adult rat cerebrocortical nerve terminals; (ii) P2X(7) receptors are localized on glutamatergic nerve terminals; (iii) P2X(7) receptors play a significant role in ATP-evoked glutamate efflux, which involves Ca(2+)-dependent vesicular release; and (iv) the P2X(7) receptor itself constitutes a significant Ca(2+)-independent mode of exit for glutamate. (literal)

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