http://www.cnr.it/ontology/cnr/individuo/prodotto/ID9649
Adult astroglia is competent for Na+/Ca2+ exchanger-mediated exocytotic glutamate release triggered by mild depolarization. (Articolo in rivista)
- Type
- Label
- Adult astroglia is competent for Na+/Ca2+ exchanger-mediated exocytotic glutamate release triggered by mild depolarization. (Articolo in rivista) (literal)
- Anno
- 2007-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1111/j.1471-4159.2007.04826.x (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Silvio Paluzzi*; Susanna Alloisio°;? Simona Zappettini*; Marco Milanese*; Luca Raiteri*^; Mario Nobile°;? Giambattista Bonanno*^ (literal)
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- *Department of Experimental Medicine, Pharmacology and Toxicology Section, University of Genoa, Genoa, Italy
°?Institute of Biophysics, National Research Council, Genoa, Italy
?^Center of Excellence for Biomedical Research, University of Genoa, Genoa, Italy (literal)
- Titolo
- Adult astroglia is competent for Na+/Ca2+ exchanger-mediated exocytotic glutamate release triggered by mild depolarization. (literal)
- Abstract
- Glutamate release induced by mild depolarization was studied in astroglial preparations from the adult rat cerebral cortex, that is acutely isolated glial sub-cellular particles (gliosomes), cultured adult or neonatal astrocytes, and neuron-conditioned astrocytes. K+ (15, 35 mmol/L), 4-aminopyridine (0.1, 1 mmol/L) or veratrine (1, 10 micromol/L) increased endogenous glutamate or [3H]D-aspartate release from gliosomes. Neurotransmitter release was partly dependent on external Ca2+, suggesting the involvement of exocytotic-like processes, and partly because of the reversal of glutamate transporters. K+ increased gliosomal membrane potential, cytosolic Ca2+ concentration [Ca2+]i, and vesicle fusion rate. Ca2+ entry into gliosomes and glutamate release were independent from voltage-sensitive Ca2+ channel opening; they were instead abolished by 2-[2-[4-(4-nitrobenzyloxy)phenyl]ethyl]isothiurea (KB-R7943), suggesting a role for the Na+/Ca2+ exchanger working in reverse mode. K+ (15, 35 mmol/L) elicited increase of [Ca2+]i and Ca2+-dependent endogenous glutamate release in adult, not in neonatal, astrocytes in culture. Glutamate release was even more marked in in vitro neuron-conditioned adult astrocytes. As seen for gliosomes, K+-induced Ca2+ influx and glutamate release were abolished by KB-R7943 also in cultured adult astrocytes. To conclude, depolarization triggers in vitro glutamate exocytosis from in situ matured adult astrocytes; an aptitude grounding on Ca2+ influx driven by the Na+/Ca2+ exchanger working in the reverse mode. (literal)
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