The potassium channel KAT1 is activated by plant and animal 14-3-3 proteins. (Articolo in rivista)

Type
Label
  • The potassium channel KAT1 is activated by plant and animal 14-3-3 proteins. (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Alternative label
  • Sottocornola B, S. Visconti, S. Orsi, S. Gazzarrini, S. Giacometti, C. Olivari, A. Abenavoli, L. Camoni, P. Aducci, M. Marra, G. Thiel and A. Moroni. (2006)
    The potassium channel KAT1 is activated by plant and animal 14-3-3 proteins.
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Sottocornola B, S. Visconti, S. Orsi, S. Gazzarrini, S. Giacometti, C. Olivari, A. Abenavoli, L. Camoni, P. Aducci, M. Marra, G. Thiel and A. Moroni. (literal)
Pagina inizio
  • 35735 (literal)
Pagina fine
  • 35741 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 281 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 65. 2006. J Biol Chem. 2006 281(47):35735-41 (literal)
Titolo
  • The potassium channel KAT1 is activated by plant and animal 14-3-3 proteins. (literal)
Abstract
  • 14-3-3 proteins modulate the plant inward rectifier K+ channel KAT1 heterologously expressed in Xenopus oocytes. Injection of recombinant plant 14-3-3 proteins into oocytes shifted the activation curve of KAT1 by +11 mV and increased the tau(on). KAT1 was also modulated by 14-3-3 proteins of Xenopus oocytes. Titration of the endogenous 14-3-3 proteins by injection of the peptide Raf 621p resulted in a strong decrease in KAT1 current (approximately 70% at -150 mV). The mutation K56E performed on plant protein 14-3-3 in a highly conserved recognition site prevented channel activation. Because the maximal conductance of KAT1 was unaffected by 14-3-3, we can exclude that they act by increasing the number of channels, thus ruling out any effect of these proteins on channel trafficking and/or insertion into the oocyte membrane. 14-3-3 proteins also increased KAT1 current in inside-out patches, suggesting a direct interaction with the channel. Direct interaction was confirmed by overlay experiments with radioactive 14-3-3 on oocyte membranes expressing KAT1. (literal)
Prodotto di
Autore CNR

Incoming links:


Prodotto
Autore CNR di
data.CNR.it