Nickel differentially affects NMDA receptor channels in developing cultured rat neurons (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Nickel differentially affects NMDA receptor channels in developing cultured rat neurons (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.brainres.2006.01.059 (literal)

Alternative label

• Paola Gavazzo; Monica Mazzolini; Mariateresa Tedesco; Carla Marchetti (2006)
  Nickel differentially affects NMDA receptor channels in developing cultured rat neurons
  in Brain research
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Paola Gavazzo; Monica Mazzolini; Mariateresa Tedesco; Carla Marchetti (literal)

Pagina inizio

• 71 (literal)

Pagina fine

• 79 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.sciencedirect.com/science/article/pii/S0006899306001740 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 1078 (literal)

Rivista

• Brain research (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 1 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Biofisica, Consiglio Nazionale delle Ricerche, via De Marini, 6, 16149 Genova, Italy DIBE, Università di Genova, Italy (literal)

Titolo

• Nickel differentially affects NMDA receptor channels in developing cultured rat neurons (literal)

Abstract

• Nickel (Ni(2+)) is a transition metal that exerts multiple and complex effects on N-methyl-d-aspartate (NMDA) channels. In both HEK293 cells and Xenopus laevis oocytes expressing recombinant NMDA receptors, Ni(2+) (<100 microM) caused a potentiation of NR2B-containing channels but a voltage-independent inhibition in those containing NR2A. We took advantage of this different response to investigate the developmental switch between NR2B and NR2A subunits in neonatal rat cerebellar granule cells up to 16 days in vitro (DIV) and in rat embryo cortical neurons up to 35 DIV. In both cultures, the effect of Ni(2+) on the NMDA current gradually changed from potentiating to inhibitory with progressing DIV, and the decline of potentiation correlated well with the decrease in sensitivity for the NR2B specific antagonist ifenprodil. Dose-dependent experiments confirmed that Ni(2+) has a different effect in younger cultures with respect to older ones, in agreement with an increase of the percentage of NR2A-containing receptors. The developmental switch occurred within the first 5 DIV in cerebellar granule cells and after 20 DIV in cortical neurons. All these data indicate that Ni(2+) is a suitable marker for the identification of NR2A and NR2B native channel subunits and can be used to trace the development of NMDA receptor composition. (literal)

Prodotto di

• Meccanismi molecolari della permeabilità di membrana (MD.P01.009.001) (Modulo)
• Istituto di biofisica (IBF) (Istituto)

Autore CNR

• CARLA MARCHETTI (Unità di personale interno)
• PAOLA GAVAZZO (Unità di personale interno)

Incoming links:

Autore CNR di

• CARLA MARCHETTI (Unità di personale interno)
• PAOLA GAVAZZO (Unità di personale interno)

Prodotto

• Istituto di biofisica (IBF) (Istituto)
• Meccanismi molecolari della permeabilità di membrana (MD.P01.009.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Brain research (Rivista)