Modulation of Reactive Oxygen Species Production During Osmotic Stress inArabidopsis thaliana Cultured Cells: Involvement of the Plasma MembraneCa2+-ATPase and H+-ATPase. (Articolo in rivista)

Type
Label
  • Modulation of Reactive Oxygen Species Production During Osmotic Stress inArabidopsis thaliana Cultured Cells: Involvement of the Plasma MembraneCa2+-ATPase and H+-ATPase. (Articolo in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Alternative label
  • Beffagna N., Buffoli B., Busi C. (2005)
    Modulation of Reactive Oxygen Species Production During Osmotic Stress inArabidopsis thaliana Cultured Cells: Involvement of the Plasma MembraneCa2+-ATPase and H+-ATPase.
    in Plant and Cell Physiology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Beffagna N., Buffoli B., Busi C. (literal)
Pagina inizio
  • 1236 (literal)
Pagina fine
  • 1339 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 46 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Biofisica del CNR, Sezione di Milan; Dipartimento di Biologia, Università degli Studi di Milano (literal)
Titolo
  • Modulation of Reactive Oxygen Species Production During Osmotic Stress inArabidopsis thaliana Cultured Cells: Involvement of the Plasma MembraneCa2+-ATPase and H+-ATPase. (literal)
Abstract
  • In Arabidopsis thaliana cells, hypoosmotic treatment initially stimulates Ca2+ influx and inhibits its efflux and, concurrently, promotes a large H2O2 accumulation in the external medium, representative of reactive oxygen species (ROS) production. After the first 10-15 min, Ca2+ influx rate is, however, lowered, and a large rise in Ca2+ efflux, concomitant with a rapid decline in H2O2 level, takes place. The drop of the H2O2 peak, as well as the efflux of Ca2+, are prevented by treatment with submicromolar concentrations of eosin yellow (EY), selectively inhibiting the Ca2+-ATPase of the plasma membrane (PM). Comparable changes of Ca2+ fluxes are also induced by hyperosmotic treatment. However, in this case, the H2O2 level does not rise, but declines below control levels when Ca2+ efflux is activated. Also K+ and H+ net fluxes across the PM and cytoplasmic pH (pH(cyt)) are very differently influenced by the two opposite stresses: strongly decreased by hypoosmotic stress and increased under hyperosmotic treatment. The H2O2 accumulation kinetics, followed as a function of the pH(cyt) changes imposed by modulation of the PM H+-ATPase activity or weak acid treatment, show a close correlation between pH(cyt) and H2O2 formed, a larger amount being produced for changes towards acidic pH values. Overall, these results confirm a relevant role for the PM Ca2+-ATPase in switching off the signal triggering ROS production, and propose a role for the PM H+-ATPase in modulating the development of the oxidative wave through the pH(cyt) changes following the changes of its activity induced by stress conditions. (literal)
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