Ion transport and metal sensitivity of vacuolar channels from the roots of the aquatic plant Eichhornia crassipes (Articolo in rivista)

Type
Label
  • Ion transport and metal sensitivity of vacuolar channels from the roots of the aquatic plant Eichhornia crassipes (Articolo in rivista) (literal)
Anno
  • 2001-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1046/j.1365-3040.2001.00777.x (literal)
Alternative label
  • Paganetto A. 1, Carpaneto A. 1, Gambale F. 1 (2001)
    Ion transport and metal sensitivity of vacuolar channels from the roots of the aquatic plant Eichhornia crassipes
    in Plant, cell and environment (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Paganetto A. 1, Carpaneto A. 1, Gambale F. 1 (literal)
Pagina inizio
  • 1329 (literal)
Pagina fine
  • 1336 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 24 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1: CNR (literal)
Titolo
  • Ion transport and metal sensitivity of vacuolar channels from the roots of the aquatic plant Eichhornia crassipes (literal)
Abstract
  • Using the patch-clamp technique, we investigated the transport properties of vacuolar ion channels from the roots of water hyacinth, Eichhornia crassipes (Mart. Solms, Pontederiacae). Eichhornia crassipes vacuoles displayed large voltage-dependent rectifying slow-vacuolar (SV) currents, which activated in a few seconds at positive potentials and deactivated at negative voltages in a few hundreds of millseconds. Similarly to SV channel previously identified in the tonoplast of terrestrial plants, SV currents in E. crassipes were activated by micromolar concentrations of Ca2+ and current slightly increased (25%) on addition (10 mm) of the reducing agent dithiothreitol (DTT). Eichhornia crassipes SV channels were equally permeable to K+ and Na+. The permeability sequence derived from current values is: K+ Na+ > Rb+ > NH4+ Cs+ >> TEA+. Excised membrane patches displayed single channel transitions typical of SV-type single channel openings with a conductance of (83·0 ± 5·6) pS; a smaller channel with a conductance of (31·0 ± 2·7) pS was also identified. Metals such as Ni2+ and Zn2+ decreased the vacuolar current in a reversible manner. However, although Zn2+ inhibition is comparable to that induced by the same metal in vacuoles from the main root of sugar beet (Beta vulgaris L.), the inhibition of the SV currents by Ni2+ is not as substantial in E. crassipes as in sugar beet. To our knowledge, this is the first electrophysiological characterization of ionic transport in E. crassipes, a pervasive troublesome aquatic weed, which has exceptional absorption properties of several water contaminants such as heavy metals, pesticides and phenols. (literal)
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