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Lead inhibition of NMDA channels in native and recombinant receptors. (Articolo in rivista)
- Type
- Label
- Lead inhibition of NMDA channels in native and recombinant receptors. (Articolo in rivista) (literal)
- Anno
- 2001-01-01T00:00:00+01:00 (literal)
- Alternative label
Gavazzo P. , Gazzoli A. , Mazzolini M. , Marchetti C. (2001)
Lead inhibition of NMDA channels in native and recombinant receptors.
in NeuroReport (Oxf.)
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- Gavazzo P. , Gazzoli A. , Mazzolini M. , Marchetti C. (literal)
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- ISI Web of Science (WOS) (literal)
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- Titolo
- Lead inhibition of NMDA channels in native and recombinant receptors. (literal)
- Abstract
- NMDA channels are key targets for lead (Pb2+) neurotoxicity and Pb2+-induced inhibition of NMDA current is age- and subunit-dependent. In rat cerebellar granule cells maintained in high KCl, glycine affinity as well as sensitivity to ifenprodil change significantly with the days in vitro, indicating a reduction of NR2B subunit expression. Pb2+ blocked NMDA current with IC50 approximately 4 microM and this effect decreased significantly during the second week in vitro. In Xenopus laevis oocytes expressing recombinant NR1-NR2A, NR1-NR2B or NR1-NR2C receptors, Pb2+ inhibited glutamate-activated currents with IC50 of 3.3, 2.5 and 4.7 microM respectively. These data indicate that Pb2+ action is dependent on subunit composition and suggest that down-regulation of the NR2B subunit is correlated to a diminished sensitivity to Pb2+ inhibition. (literal)
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