http://www.cnr.it/ontology/cnr/individuo/prodotto/ID9280
Involvement of the plasma membrane Ca2+?ATPase in the short term response of Arabidopsis thaliana cultured cells to oligogalacturonides. (Articolo in rivista)
- Type
- Label
- Involvement of the plasma membrane Ca2+?ATPase in the short term response of Arabidopsis thaliana cultured cells to oligogalacturonides. (Articolo in rivista) (literal)
- Anno
- 2004-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1055/s-2004-817848 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Romani G.; Bonza M.C.; Filippini I.; Cerana M.; Beffagna N.; De Michelis, M.I. (literal)
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- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Romani G., Beffagna N., De Michelis, M.I., IBF CNR Milano; Bonza M.C., Filippini I., Cerana M., De Michelis, M.I., Dipartimento di Biologia Univ. degli Studi di Milano. (literal)
- Titolo
- Involvement of the plasma membrane Ca2+?ATPase in the short term response of Arabidopsis thaliana cultured cells to oligogalacturonides. (literal)
- Abstract
- Treatment of Arabidopsis thaliana cells with oligogalacturonides (OG) initiates a transient production of reactive oxygen species (ROS), the concentration of which in the medium peaks after about 20 min of treatment. The analysis of OG effects on Ca (2+) fluxes shows that OG influence both Ca (2+) influx and Ca (2+) efflux (measured as (45)Ca (2+) fluxes) in a complex way. During the first 10 - 15 min, OG stimulate Ca (2+) influx and decrease its efflux, while at successive times of treatment, OG cause an increase of Ca (2+) efflux and a slight decrease of its influx. Treatment with sub- micro M concentrations of eosin yellow (EY), which selectively inhibits the Ca (2+)-ATPase of plasma membrane (PM), completely prevents the OG-induced increase in Ca (2+) efflux. EY also suppresses the transient feature of OG-induced ROS accumulation, keeping the level of ROS in the medium high. The biochemical analysis of PM purified from OG-treated cells indicates that treatment with OG for 15 to 45 min induces a significant decrease in Ca (2+)-ATPase activation by exogenous calmodulin (CaM), and markedly increases the amount of CaM associated with the PM. During the same time span, OG do not influence the expression of At-ACA8, the main isoform of PM Ca (2+)-ATPase in suspension-cultured A. thaliana cells, and of CaM genes. Overall, the reported results demonstrate that the PM Ca (2+)-ATPase is involved in the response of plant cells to OG and is essential in regulation of the oxidative burst. (literal)
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