Involvement of the plasma membrane Ca2+?ATPase in the short term response of Arabidopsis thaliana cultured cells to oligogalacturonides. (Articolo in rivista)

Type
Label
  • Involvement of the plasma membrane Ca2+?ATPase in the short term response of Arabidopsis thaliana cultured cells to oligogalacturonides. (Articolo in rivista) (literal)
Anno
  • 2004-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1055/s-2004-817848 (literal)
Alternative label
  • Romani G.; Bonza M.C.; Filippini I.; Cerana M.; Beffagna N.; De Michelis, M.I. (2004)
    Involvement of the plasma membrane Ca2+?ATPase in the short term response of Arabidopsis thaliana cultured cells to oligogalacturonides.
    in Plant biology (Stuttg.)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Romani G.; Bonza M.C.; Filippini I.; Cerana M.; Beffagna N.; De Michelis, M.I. (literal)
Pagina inizio
  • 192 (literal)
Pagina fine
  • 200 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 6 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 2 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Romani G., Beffagna N., De Michelis, M.I., IBF CNR Milano; Bonza M.C., Filippini I., Cerana M., De Michelis, M.I., Dipartimento di Biologia Univ. degli Studi di Milano. (literal)
Titolo
  • Involvement of the plasma membrane Ca2+?ATPase in the short term response of Arabidopsis thaliana cultured cells to oligogalacturonides. (literal)
Abstract
  • Treatment of Arabidopsis thaliana cells with oligogalacturonides (OG) initiates a transient production of reactive oxygen species (ROS), the concentration of which in the medium peaks after about 20 min of treatment. The analysis of OG effects on Ca (2+) fluxes shows that OG influence both Ca (2+) influx and Ca (2+) efflux (measured as (45)Ca (2+) fluxes) in a complex way. During the first 10 - 15 min, OG stimulate Ca (2+) influx and decrease its efflux, while at successive times of treatment, OG cause an increase of Ca (2+) efflux and a slight decrease of its influx. Treatment with sub- micro M concentrations of eosin yellow (EY), which selectively inhibits the Ca (2+)-ATPase of plasma membrane (PM), completely prevents the OG-induced increase in Ca (2+) efflux. EY also suppresses the transient feature of OG-induced ROS accumulation, keeping the level of ROS in the medium high. The biochemical analysis of PM purified from OG-treated cells indicates that treatment with OG for 15 to 45 min induces a significant decrease in Ca (2+)-ATPase activation by exogenous calmodulin (CaM), and markedly increases the amount of CaM associated with the PM. During the same time span, OG do not influence the expression of At-ACA8, the main isoform of PM Ca (2+)-ATPase in suspension-cultured A. thaliana cells, and of CaM genes. Overall, the reported results demonstrate that the PM Ca (2+)-ATPase is involved in the response of plant cells to OG and is essential in regulation of the oxidative burst. (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Prodotto
Autore CNR di
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
Insieme di parole chiave di
data.CNR.it