http://www.cnr.it/ontology/cnr/individuo/prodotto/ID9212
Anticonvulsant phenytoin affects voltage-gated potassium currents in cerebellar granule cells (Articolo in rivista)
- Type
- Label
- Anticonvulsant phenytoin affects voltage-gated potassium currents in cerebellar granule cells (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Pisciotta M., Prestipino G. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Titolo
- Anticonvulsant phenytoin affects voltage-gated potassium currents in cerebellar granule cells (literal)
- Abstract
- The anticonvulsant drug phenytoin (diphenylhydantoin, DPH) was examined
for its action on potassium currents in cerebellar granule cells using the
whole-cell patch-clamp technique. Granular cells expressed two main types
of voltage-dependent potassium currents: the first, sensitive to
Tetraethylammonium ion (TEA), resembles a delayed rectifier K(+) channel (I
(d)); the second shows biophysical and pharmacological properties similar
to an I(A)-type potassium current. Phenytoin blocks the I(A) current in a
dose-dependent manner, with an apparent dissociation constant K(d) of
(73+/-7) microM. The drug shifts the steady-state inactivation curves
towards a more negative potential, stabilizing the inactivated state,
while the activation kinetics remain unaffected. The estimated K(d) when
the cell is held to -100 mV (closed state of the channel) is 145+/-8
microM which decreases to 35+/-10 microM at -80 mV holding potential
(partial inactivation of the channel). Phenytoin shows a discriminant
behaviour between the two different types of potassium channels because at
high concentration the effect of the drug on the delayed rectifier K(+)
channel is negligible.
(literal)
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