http://www.cnr.it/ontology/cnr/individuo/prodotto/ID9204
A long leader intron of the Ostub 16 rice B-tubulin gene is required fir high level gene expression and can autonomously promote transcription both in vivo and in vitro. (Articolo in rivista)
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- A long leader intron of the Ostub 16 rice B-tubulin gene is required fir high level gene expression and can autonomously promote transcription both in vivo and in vitro. (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
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Morello L., Bardini M., Sala F., Breviario D. (2002)
A long leader intron of the Ostub 16 rice B-tubulin gene is required fir high level gene expression and can autonomously promote transcription both in vivo and in vitro.
in Plant journal (Print)
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- Morello L., Bardini M., Sala F., Breviario D. (literal)
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- Titolo
- A long leader intron of the Ostub 16 rice B-tubulin gene is required fir high level gene expression and can autonomously promote transcription both in vivo and in vitro. (literal)
- Abstract
- A 2 kb DNA fragment, upstream of the rice beta-tubulin isotype 16
(Ostub16) coding sequence, was isolated using inverse PCR and screening of
a tubulin-enriched lambda library. An intron (863 bp) present in the 5'
untranslated region (5' UTR) is spliced out to produce the most abundant
mRNA species which corresponds to the previously cloned Ostub16 cDNA.
Transient expression assays performed on rice embryogenic calluses with
chimeric Ostub16::GUS constructs demonstrated that the entire 2 kb
upstream sequence has a strong promoter activity, and that the 863 bp
intron is required for high-level GUS expression. In addition, the intron
sequence is capable per se of sustaining a weak but consistent GUS
expression. Two rare Ostub16 transcripts, with a start site mapping within
this intron sequence, were detected in rice coleoptile cells. The
transcription start site mapped at position 290 with respect to the ATG
codon, and the shorter molecule originated from splicing of the same
precursor mRNA. Therefore transcriptional expression of rice beta-tubulin
isotype 16 results in the synthesis of two premRNA molecules (I and II)
encoding for three different mRNA species. We discuss these findings in
terms of function and molecular evolution of the mechanisms that control
plant -tubulin gene expression. (literal)
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