The phytotoxic lipodepsipeptide syringopeptin 25A from Pseudomonas syringae pv syringae forms ion channels in sugar beet vacuoles (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• The phytotoxic lipodepsipeptide syringopeptin 25A from Pseudomonas syringae pv syringae forms ion channels in sugar beet vacuoles (Articolo in rivista) (literal)

Anno

• 2002-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s00232-001-0187-x (literal)

Alternative label

• Carpaneto A., Dalla Serra M., Menestrina G., Fogliano V., Gambale F. (2002)
  The phytotoxic lipodepsipeptide syringopeptin 25A from Pseudomonas syringae pv syringae forms ion channels in sugar beet vacuoles
  in The journal of membrane biology (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Carpaneto A., Dalla Serra M., Menestrina G., Fogliano V., Gambale F. (literal)

Pagina inizio

• 1 (literal)

Pagina fine

• 12 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 188 (literal)

Rivista

• ?The ?journal of membrane biology (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR, Ist Biofis, I-16149 Genoa, Italy CNR, ITC, IBF, Ctr Fis Stati Aggregati, I-38050 Povo, Trento, Italy Univ Naples Federico II, Dipartimento Sci Alimenti, I-80138 Naples, Italy (literal)

Titolo

• The phytotoxic lipodepsipeptide syringopeptin 25A from Pseudomonas syringae pv syringae forms ion channels in sugar beet vacuoles (literal)

Abstract

• Syringopeptin 25A (SP(25)A) belongs to a family of cyclic lipodepsipeptides (LDPs) produced by the gram-negative bacterium Pseudomonas syringae, a phytopathogenic organism that affects several plants of agronomic interest. LDPs increase the permeability of plasma and, possibly, intracellular membranes in plant cells. Consistently, SP (25)A forms ion channels in planar lipid bilayers and other model membranes. Here we used sugar beet tonoplasts as a new biological model system to study toxin action. When applied to the vacuoles by a fast perfusion procedure, SP(25)A increases membrane permeability by forming discrete ion channels even at low applied potentials. The SP(25)A channel displays anion selectivity (with a Cl-/K+ permeability ratio of 6.7 +/- 1.3) and has intrinsic rectification properties that derive from a different channel conductance at negative and positive voltages, presumably owing to an asymmetric distribution of fixed charges on the pore. Substitution of chloride with different anions reveals the following selectivity sequence NO3- approximately Cl-> F- > gluconate-, suggesting that the permeation pore is filled with water. The properties of the SP(25)A channels in vacuolar membranes are similar to those observed in planar lipid membranes prepared with asolectin. This work provides a direct demonstration of toxin effects on a native plant membrane, extending to a biological system previous results obtained on artificial planar lipid membranes. (literal)

Prodotto di

• Istituto di biofisica (IBF) (Istituto)

Autore CNR

• FRANCO GAMBALE (Persona)
• GIANFRANCO MENESTRINA (Unità di personale interno)
• MAURO DALLA SERRA (Unità di personale interno)
• ARMANDO CARPANETO (Unità di personale interno)

Incoming links:

Autore CNR di

• ARMANDO CARPANETO (Unità di personale interno)
• FRANCO GAMBALE (Persona)
• MAURO DALLA SERRA (Unità di personale interno)
• GIANFRANCO MENESTRINA (Unità di personale interno)

Prodotto

• Istituto di biofisica (IBF) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• ?The ?journal of membrane biology (Rivista)