Improving the promiscuous nerve agent hydrolase activity of a thermostable archaeal lactonase (Articolo in rivista)

Type
Label
  • Improving the promiscuous nerve agent hydrolase activity of a thermostable archaeal lactonase (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.biortech.2010.06.102 (literal)
Alternative label
  • Merone L.; Mandrich L.; Porzio E.; Rossi M.; Müller S.; Reiter G.; Worek F.; Manco G. (2010)
    Improving the promiscuous nerve agent hydrolase activity of a thermostable archaeal lactonase
    in Bioresource technology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Merone L.; Mandrich L.; Porzio E.; Rossi M.; Müller S.; Reiter G.; Worek F.; Manco G. (literal)
Pagina inizio
  • 9204 (literal)
Pagina fine
  • 9212 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 101 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 23 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Via P. Castellino 111, 80131 Naples, Italy Bundeswehr Institute of Pharmacology and Toxicology, Neuherbergstrasse 11, 80937 Munich, Germany (literal)
Titolo
  • Improving the promiscuous nerve agent hydrolase activity of a thermostable archaeal lactonase (literal)
Abstract
  • The thermostable Phosphotriesterase-Like Lactonase from Sulfolobus solfataricus (SsoPox) hydrolyzes lactones and, at a lower rate, neurotoxic organophosphorus compounds. The persistent demand of detoxification tools in the field of agricultural wastes and restoring of conditions after terrorist acts prompted us to exploit SsoPox as a \"starter\" to evolve its ancillary nerve agents hydrolytic capability. A directed evolution strategy yielded, among several variants, the single mutant W263F with k(cat) and specificity constant against paraoxon 16- and 6-fold enhanced, respectively, compared to the wild type. Furthermore, a phenomenon of enzyme activation by SDS has been observed, which allowed to increase those values 150- and 28-fold, respectively. The activity of SsoPox against the deadly nerve gas Cyclosarin has been reported for the first time and proved to be substantially unaffected for variant W263F. Finally, outperforming efficiency of W263F was demonstrated, under severe stressing conditions, with respect to the best known phosphotriesterase PTE from Brevundimonas diminuta. (literal)
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