A new archaeal beta-glycosidase from Sulfolobus solfataricus: Seeding a novel retaining beta-glycan-specific glycoside hydrolase family along with the human non-lysosomal glucosylceramidase GBA2 (Articolo in rivista)

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  • A new archaeal beta-glycosidase from Sulfolobus solfataricus: Seeding a novel retaining beta-glycan-specific glycoside hydrolase family along with the human non-lysosomal glucosylceramidase GBA2 (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1074/jbc.M109.086470 (literal)
Alternative label
  • Cobucci-Ponzano B.; Aurilia V.; Riccio G.; Henrissat B.; Coutinho P.M.; Strazzulli A.; Padula A.; Corsaro M.M.; Pieretti G.; Pocsfalvi G.; Fiume I.; Cannio R.; Rossi M.; Moracci M. (2010)
    A new archaeal beta-glycosidase from Sulfolobus solfataricus: Seeding a novel retaining beta-glycan-specific glycoside hydrolase family along with the human non-lysosomal glucosylceramidase GBA2
    in The Journal of biological chemistry (Print); American society for biochemistry and molecular biology, Baltimore (Stati Uniti d'America)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Cobucci-Ponzano B.; Aurilia V.; Riccio G.; Henrissat B.; Coutinho P.M.; Strazzulli A.; Padula A.; Corsaro M.M.; Pieretti G.; Pocsfalvi G.; Fiume I.; Cannio R.; Rossi M.; Moracci M. (literal)
Pagina inizio
  • 20691 (literal)
Pagina fine
  • 20703 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 285 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 27 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Institute of Protein Biochemistry, CNR, Via P. Castellino 111, 80131, Naples, Italy Architecture et Fonction des Macromolecules Biologiques, UMR6098, CNRS, Universites Aix-Marseille I & II, 163 Avenue de Luminy, 13288 Marseille, France Dipartimento di Chimica Organica e Biochimica and the ?Dipartimento di Biologia Strutturale e Funzionale, Universita` di Napoli Federico II, Complesso Universitario di Monte S. Angelo, Via Cinthia 4, 80126 Naples, Italy (literal)
Titolo
  • A new archaeal beta-glycosidase from Sulfolobus solfataricus: Seeding a novel retaining beta-glycan-specific glycoside hydrolase family along with the human non-lysosomal glucosylceramidase GBA2 (literal)
Abstract
  • Carbohydrate active enzymes (CAZymes) are a large class of enzymes, which build and breakdown the complex carbohydrates of the cell. On the basis of their amino acid sequences they are classified in families and clans that show conserved catalytic mechanism, structure, and active site residues, but may vary in substrate specificity. We report here the identification and the detailed molecular characterization of a novel glycoside hydrolase encoded from the gene sso1353 of the hyperthermophilic archaeon Sulfolobus solfataricus. This enzyme hydrolyses aryl beta-gluco- and beta-xylosides and the observation of transxylosylation reactions products demonstrates that SSO1353 operates via a retaining reaction mechanism. The catalytic nucleophile (Glu335) was identified through trapping of the 2-deoxy-2-fluoroglucosyl-enzyme intermediate and subsequent peptide mapping, while the general acid/base was identified as Asp462 through detailed mechanistic analysis of a mutant at that position, including azide rescue experiments. SSO1353 has detectable homologs of unknown specificity among Archaea, Bacteria and Eukarya and shows distant similarity to the non-lysosomal bile acid beta-glucosidase GBA2 also known as glucocerebrosidase. On the basis of our findings we propose that SSO1353 and its homologs are classified in a new CAZy family, named GH116, which so far includes beta-glucosidases (EC 3.2.1.21), beta-xylosidases (EC 3.2.1.37), and glucocerebrosidases (EC 3.2.1.45) as known enzyme activities. (literal)
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